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<p>The in vitro propagation of <i>Rosa hybrida</i> ‘White Beauty’ is often hindered by physiological disorders such as shoot-tip necrosis and leaf chlorosis, primarily attributed to ethylene accumulation in sealed culture vessels. This study aimed to evaluate the effectiveness of three ethylene inhibitors—silver nitrate, aminoethoxyvinylglycine (AVG), and silver nanoparticles (AgNPs)—in alleviating these disorders and improving plant quality. Treatment with 3.0&#xa0;mg L<sup>−1</sup> AgNO<sub>3</sub> and 2.0&#xa0;mg L<sup>−1</sup> AgNPs significantly reduced shoot-tip necrosis and leaf chlorosis, while enhancing shoot growth and soil plant analysis development (SPAD) values. While AVG treatments reduced necrosis, their impact on plant vigor was limited. The role of ethylene in shoot-tip necrosis and leaf chlorosis was confirmed through co-application with 1-aminocyclopropane-1-carboxylic acid (ACC), an ethylene precursor. AgNPs effectively reversed ACC-induced shoot-tip necrosis and leaf chlorosis. Additionally, treatment with 0.9 and 1.8&#xa0;mg L<sup>−1</sup> indole-3-acetic acid (IAA) significantly improved the rooting percentage, root number, and post-acclimatization growth, indicating enhanced root system architecture and transplant performance. These results demonstrate that the use of AgNO<sub>3</sub> or AgNPs with IAA resulted in the optimal, high-quality micropropagation of <i>Rosa hybrida</i> ‘White Beauty.’ This strategy holds promise for commercial-scale production and warrants further investigation into the mechanisms underlying hormonal signaling and the field performance of regenerated plantlets.</p>

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Roles of ethylene inhibitors in mitigating shoot tip necrosis and enhancing the growth of Rosa hybrida ‘White beauty’

  • 期刊论文

  • 发表日期：

    2026年3月9日

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  • Dongyeon Seo,
  • Aung Htay Naing,
  • Youn Jung Choi,
  • Mi Young Chung,
  • Chang Kil Kim

摘要

The in vitro propagation of Rosa hybrida ‘White Beauty’ is often hindered by physiological disorders such as shoot-tip necrosis and leaf chlorosis, primarily attributed to ethylene accumulation in sealed culture vessels. This study aimed to evaluate the effectiveness of three ethylene inhibitors—silver nitrate, aminoethoxyvinylglycine (AVG), and silver nanoparticles (AgNPs)—in alleviating these disorders and improving plant quality. Treatment with 3.0 mg L−1 AgNO3 and 2.0 mg L−1 AgNPs significantly reduced shoot-tip necrosis and leaf chlorosis, while enhancing shoot growth and soil plant analysis development (SPAD) values. While AVG treatments reduced necrosis, their impact on plant vigor was limited. The role of ethylene in shoot-tip necrosis and leaf chlorosis was confirmed through co-application with 1-aminocyclopropane-1-carboxylic acid (ACC), an ethylene precursor. AgNPs effectively reversed ACC-induced shoot-tip necrosis and leaf chlorosis. Additionally, treatment with 0.9 and 1.8 mg L−1 indole-3-acetic acid (IAA) significantly improved the rooting percentage, root number, and post-acclimatization growth, indicating enhanced root system architecture and transplant performance. These results demonstrate that the use of AgNO3 or AgNPs with IAA resulted in the optimal, high-quality micropropagation of Rosa hybrida ‘White Beauty.’ This strategy holds promise for commercial-scale production and warrants further investigation into the mechanisms underlying hormonal signaling and the field performance of regenerated plantlets.