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<p>Chloroplasts are essential organelles responsible for photosynthesis and play a central role in determining plant growth and productivity. Here, we investigated the functional role of <i>OsPRIN2</i> in chloroplast development in rice. CRISPR/Cas9-generated <i>osprin2</i> knock-out mutants exhibited an albino, seedling-lethal phenotype. Analyses of plastid morphology revealed that loss of <i>OsPRIN2</i> causes severe defects in chloroplast formation, which was further supported by protoplast observations showing a complete absence of chloroplasts in <i>osprin2</i> knock-out rice. In addition, the <i>OsPRIN2</i> knock-out mutation specifically suppressed the expression of chloroplast genes tightly regulated by the plastid-encoded RNA polymerase. These findings indicate that <i>OsPRIN2</i> is essential for chloroplast formation in rice through the regulation of PEP-dependent transcription. Furthermore, we also found that <i>OsPRIN2</i> coordinates with <i>OsTRXz</i>, a chloroplastic thioredoxin that mediates PEP activity and chloroplast development in rice. Collectively, these results suggest that <i>OsPRIN2</i> regulates chloroplast formation in rice by controlling PEP-dependent expression of chloroplastic genes, and that the coordination between <i>OsPRIN2</i> and <i>OsTRXz</i> is deeply involved in this process.</p>

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PLASTID REDOX INSENSITIVE 2 is essential for chloroplast development in rice

  • 期刊论文

  • 发表日期：

    2026年2月24日

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  • Jinhyeong Kim,
  • Geupil Jang

摘要

Chloroplasts are essential organelles responsible for photosynthesis and play a central role in determining plant growth and productivity. Here, we investigated the functional role of OsPRIN2 in chloroplast development in rice. CRISPR/Cas9-generated osprin2 knock-out mutants exhibited an albino, seedling-lethal phenotype. Analyses of plastid morphology revealed that loss of OsPRIN2 causes severe defects in chloroplast formation, which was further supported by protoplast observations showing a complete absence of chloroplasts in osprin2 knock-out rice. In addition, the OsPRIN2 knock-out mutation specifically suppressed the expression of chloroplast genes tightly regulated by the plastid-encoded RNA polymerase. These findings indicate that OsPRIN2 is essential for chloroplast formation in rice through the regulation of PEP-dependent transcription. Furthermore, we also found that OsPRIN2 coordinates with OsTRXz, a chloroplastic thioredoxin that mediates PEP activity and chloroplast development in rice. Collectively, these results suggest that OsPRIN2 regulates chloroplast formation in rice by controlling PEP-dependent expression of chloroplastic genes, and that the coordination between OsPRIN2 and OsTRXz is deeply involved in this process.