Scholarlink

<p>Regeneration of viable shoots from tissue explants is critical for producing genetically engineered plants. However, in most pepper (<i>Capsicum annuum</i>) genotypes, non-viable rather than viable shoots are frequently regenerated, which limits the development of improved cultivars through modern biotechnology. In this study, we established a high-efficiency method for generating viable transgenic shoots by suppressing microRNA396 activity in pepper. Cotyledon explants were cultured on two different shoot-inducing media (M1 and M2) after introducing STTM396a, a target-mimicry artificial RNA designed to suppress microRNA396 function. Ectopic expression of <i>STTM396a</i> in T0 explants increased callus formation, reaching up to 45%. Shoot regeneration occurred only in explants cultured on M2 medium, and STTM396a did not improve the overall regeneration frequency. Notably, the rooting rate of regenerated shoots increased approximately 4.1-fold in the STTM396a group, demonstrating that STTM396a promotes the regeneration of viable shoots. Furthermore, the efficiency of producing transgenic plants increased by approximately 2.5-fold with <i>STTM396a</i> expression. These findings indicate that ectopic expression of <i>STTM396a</i>, combined with cultivation on M2 medium, enhances the efficiency of both viable shoot regeneration and transgenic plant production in pepper.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Suppressing microRNA396 activity enhances regeneration efficiency of viable shoots from cotyledon explants in pepper

  • 期刊论文

  • 发表日期：

    2026年2月7日

查看全文
  • Min Jun Shin,
  • Seong Sub Ku,
  • Su-Jin Park,
  • Sang Un Park,
  • Sung Ran Min,
  • Hyun-Soon Kim,
  • Hyo-Jun Lee

摘要

Regeneration of viable shoots from tissue explants is critical for producing genetically engineered plants. However, in most pepper (Capsicum annuum) genotypes, non-viable rather than viable shoots are frequently regenerated, which limits the development of improved cultivars through modern biotechnology. In this study, we established a high-efficiency method for generating viable transgenic shoots by suppressing microRNA396 activity in pepper. Cotyledon explants were cultured on two different shoot-inducing media (M1 and M2) after introducing STTM396a, a target-mimicry artificial RNA designed to suppress microRNA396 function. Ectopic expression of STTM396a in T0 explants increased callus formation, reaching up to 45%. Shoot regeneration occurred only in explants cultured on M2 medium, and STTM396a did not improve the overall regeneration frequency. Notably, the rooting rate of regenerated shoots increased approximately 4.1-fold in the STTM396a group, demonstrating that STTM396a promotes the regeneration of viable shoots. Furthermore, the efficiency of producing transgenic plants increased by approximately 2.5-fold with STTM396a expression. These findings indicate that ectopic expression of STTM396a, combined with cultivation on M2 medium, enhances the efficiency of both viable shoot regeneration and transgenic plant production in pepper.