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<p>Sucrose transporters (SUTs) are essential for assimilate translocation from source to sink tissues, playing a vital role in plant growth and development. While the characteristics and biological functions of <i>SUT</i> genes have been extensively studied in various higher plants, their roles in <i>Nicotiana tabacum</i> remain poorly understood. In this study, eight <i>NtSUT</i> genes were identified in tobacco, which were classified into three distinct evolutionary clades. Members within the same clade exhibited similar exon-intron structures and motif patterns. Promoter analysis revealed <i>cis</i>-acting elements involved in regulating growth, phytohormone signaling, and abiotic stress responses. Subcellular localization showed that NtSUT proteins are predominantly localized to the plasma membrane. Yeast complementation assays confirmed their functional role in sucrose transport. Expression profiling under NaCl and PEG treatments indicated that <i>NtSUT</i> genes, especially <i>NtSUT2</i>, are crucial for stress responses. Further esculin absorption experiments conducted on yeast mutants and tobacco roots confirmed the role of <i>NtSUT2</i>-mediated sucrose transport in response to abiotic stress. Our research is the first to establish a method using esculin to study sucrose transport in tobacco, confirming the role of sucrose transport in the response of tobacco to abiotic stress.</p>

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Sucrose transporters (SUTs) mediate sucrose transport and are associated with abiotic stress response in Nicotiana tabacum

  • 期刊论文

  • 发表日期：

    2026年2月6日

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  • Yangfan Miao,
  • Linggai Cao,
  • Zhiqiang Zhang,
  • Hailan Liu,
  • Jie Zhang,
  • Zhongying Ren,
  • Jie Liu,
  • Jintao Li,
  • Zhixiao Yang,
  • Shizhou Yu,
  • Daigang Yang,
  • Wei Li

摘要

Sucrose transporters (SUTs) are essential for assimilate translocation from source to sink tissues, playing a vital role in plant growth and development. While the characteristics and biological functions of SUT genes have been extensively studied in various higher plants, their roles in Nicotiana tabacum remain poorly understood. In this study, eight NtSUT genes were identified in tobacco, which were classified into three distinct evolutionary clades. Members within the same clade exhibited similar exon-intron structures and motif patterns. Promoter analysis revealed cis-acting elements involved in regulating growth, phytohormone signaling, and abiotic stress responses. Subcellular localization showed that NtSUT proteins are predominantly localized to the plasma membrane. Yeast complementation assays confirmed their functional role in sucrose transport. Expression profiling under NaCl and PEG treatments indicated that NtSUT genes, especially NtSUT2, are crucial for stress responses. Further esculin absorption experiments conducted on yeast mutants and tobacco roots confirmed the role of NtSUT2-mediated sucrose transport in response to abiotic stress. Our research is the first to establish a method using esculin to study sucrose transport in tobacco, confirming the role of sucrose transport in the response of tobacco to abiotic stress.