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<p><i>Cullen corylifolium</i> (L.) Medik (<i>Psoralea corylifolia</i> Linn. and babchi), a medicinal plant with antimicrobial and anti-inflammatory properties, is a major natural source of bakuchiol—a meroterpene phenol with high pharmaceutical and cosmetic value. However, overharvesting and limited natural resources have raised concerns regarding sustainable production. In this study, we established an in vitro tissue culture system to induce callus formation from different explants (leaf, stem, and root) of <i>C. corylifolium</i> by optimizing the type and concentration of cytokinins and auxins. Explants were cultured in Murashige and Skoog medium supplemented with various combinations of 6-benzylaminopurine (BAP), kinetin, 2,4-dichlorophenoxyacetic acid (2,4-D), and naphthaleneacetic acid. Callus induction was most effective with BAP-2,4-D combinations. Collectively, under the present culture conditions, (+)-bakuchiol accumulation in calli was negligible or below the limit of detection, despite transcriptional evidence of pathway engagement. These follow-up investigations are necessary to combine elicitor regimens, precursor supply, and process optimization with targeted LC–MS to achieve definitive identification and quantitative assessment of (+)-bakuchiol in calli. This study highlights the utility and limitations of dedifferentiated plant tissues for specialized metabolite engineering, offering a transferable workflow for meroterpenoid pathways beyond (+)-bakuchiol.</p>

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Establishment of in vitro callus culture and analysis of Bakuchiol from Cullen corylifolium

  • 期刊论文

  • 发表日期：

    2026年1月21日

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  • Na-Gyeol Hwang,
  • Chan-Woo Park,
  • Ye-Rin Kim,
  • Hyo-Jeong Kim,
  • Ji-Hyun Kim,
  • Eunbeen Shin,
  • Seon Beom Kim,
  • Yeongju Lee,
  • Dae Youn Hwang,
  • Sunho Park,
  • Eun Sang Jung,
  • Yu-Jin Kim

摘要

Cullen corylifolium (L.) Medik (Psoralea corylifolia Linn. and babchi), a medicinal plant with antimicrobial and anti-inflammatory properties, is a major natural source of bakuchiol—a meroterpene phenol with high pharmaceutical and cosmetic value. However, overharvesting and limited natural resources have raised concerns regarding sustainable production. In this study, we established an in vitro tissue culture system to induce callus formation from different explants (leaf, stem, and root) of C. corylifolium by optimizing the type and concentration of cytokinins and auxins. Explants were cultured in Murashige and Skoog medium supplemented with various combinations of 6-benzylaminopurine (BAP), kinetin, 2,4-dichlorophenoxyacetic acid (2,4-D), and naphthaleneacetic acid. Callus induction was most effective with BAP-2,4-D combinations. Collectively, under the present culture conditions, (+)-bakuchiol accumulation in calli was negligible or below the limit of detection, despite transcriptional evidence of pathway engagement. These follow-up investigations are necessary to combine elicitor regimens, precursor supply, and process optimization with targeted LC–MS to achieve definitive identification and quantitative assessment of (+)-bakuchiol in calli. This study highlights the utility and limitations of dedifferentiated plant tissues for specialized metabolite engineering, offering a transferable workflow for meroterpenoid pathways beyond (+)-bakuchiol.