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<p>Sieve element occlusion (<i>SEO</i>) genes play important roles in the interaction between plants and pathogens. However, the information and function of <i>SEO</i> genes in <i>C. sinensis</i> (<i>CsSEOs</i>) are not yet clear. In this study, bioinformatic methods were used to identify <i>CsSEOs</i>, and subsequently analyzed the gene structure, conserved motifs, and domains of CsSEOs. The phylogenetic and collinearity relationships of SEOs in <i>Arabidopsis thaliana</i>, <i>C. sinensis</i>, and <i>Vitis vinifera</i> were determined. Additionally, expression patterns of <i>CsSEOs</i> during <i>Candidatus</i> Liberibacter asiaticus (CLas) and <i>Xanthomonas citri</i> ssp. citri (Xcc) infection, <i>cis</i>-acting elements in the promoter region of <i>CsSEOs</i>, and protein–protein interaction (PPI) networks for CsSEOs were also analyzed. Results showed that there are 9 <i>CsSEOs</i> in the genome of <i>C. sinensis</i>, with significant differences in exon–intron structures among different members. CsSEOs have conserved SEO_N and SEO_C domains, while the composition of conserved motifs varies greatly. <i>CsSEOs</i> are distributed on four chromosomes of <i>C. sinensis</i>, and they exhibit certain collinearity relationships with <i>SEO</i> genes in <i>A. thaliana</i> and <i>V. vinifera</i>. Phylogenetic analysis showed that SEOs in <i>A. thaliana</i>, <i>C. sinensis</i>, and <i>V. vinifera</i> cluster into four clades, with 2 gene gain and 8 gene loss events in <i>C. sinensis</i> compared to <i>V. vinifera</i>. The expression patterns of <i>CsSEOs</i> during CLas infection varied, with up-regulation of <i>CsSEO3</i> and <i>CsSEO6</i>, while the expression of most <i>CsSEOs</i> was down-regulated during Xcc infection. There were significant differences in the distribution of cis-acting elements in the promoter region of <i>CsSEOs</i> in response to different hormones and stresses. The PPI network analysis showed that CsSEO3 and CsSEO8 are located at the core of the network regulation nodes. This study provides a foundation for in-depth analysis of the biological functions of CsSEOs and reveals potential involvement of CsSEOs in pathogen stress responses.</p>

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Identification and characterization of Citrus sinensis SEO genes in response to citrus huanglongbing and canker

  • 期刊论文

  • 发表日期：

    2025年9月8日

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  • Xinyou Wang,
  • Kewen Wu,
  • Hongli Gai,
  • Ruimin Li

摘要

Sieve element occlusion (SEO) genes play important roles in the interaction between plants and pathogens. However, the information and function of SEO genes in C. sinensis (CsSEOs) are not yet clear. In this study, bioinformatic methods were used to identify CsSEOs, and subsequently analyzed the gene structure, conserved motifs, and domains of CsSEOs. The phylogenetic and collinearity relationships of SEOs in Arabidopsis thaliana, C. sinensis, and Vitis vinifera were determined. Additionally, expression patterns of CsSEOs during Candidatus Liberibacter asiaticus (CLas) and Xanthomonas citri ssp. citri (Xcc) infection, cis-acting elements in the promoter region of CsSEOs, and protein–protein interaction (PPI) networks for CsSEOs were also analyzed. Results showed that there are 9 CsSEOs in the genome of C. sinensis, with significant differences in exon–intron structures among different members. CsSEOs have conserved SEO_N and SEO_C domains, while the composition of conserved motifs varies greatly. CsSEOs are distributed on four chromosomes of C. sinensis, and they exhibit certain collinearity relationships with SEO genes in A. thaliana and V. vinifera. Phylogenetic analysis showed that SEOs in A. thaliana, C. sinensis, and V. vinifera cluster into four clades, with 2 gene gain and 8 gene loss events in C. sinensis compared to V. vinifera. The expression patterns of CsSEOs during CLas infection varied, with up-regulation of CsSEO3 and CsSEO6, while the expression of most CsSEOs was down-regulated during Xcc infection. There were significant differences in the distribution of cis-acting elements in the promoter region of CsSEOs in response to different hormones and stresses. The PPI network analysis showed that CsSEO3 and CsSEO8 are located at the core of the network regulation nodes. This study provides a foundation for in-depth analysis of the biological functions of CsSEOs and reveals potential involvement of CsSEOs in pathogen stress responses.