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<p>In over 95 countries, pea is a vital winter legume crop known for its protein-laden seeds, which are very useful for both dietary and fodder purposes. However, modern agricultural practices and anthropogenic activities leading to climate shift have significantly threatened this valuable crop by increasing the prevalence of abiotic and biotic stresses. To address these challenges, important QTLs and genomic loci are introgressed into modern pea cultivars. We performed a thorough meta-analysis of 594 QTL-related data collected from 17 previous investigations and projected it onto a high-density consensus map based on 6175 markers. Using this method, we identified the most precise genomic areas associated with agronomic characteristics and biotic and abiotic stress tolerances. The study identified 17&#xa0;M-QTLs with confidence intervals reduced by 4.41-folds as compared to the original QTLs. Genome-wide association (GWA) studies validated the seven M-QTLs (M-QTL1.1, 2.1, 3.1, 4.2, 5.1, 7.1, and 7.3) through their physical positions distributed over six linkage groups, which appeared to be beneficial genomic regions for capitalization by various breeding methodologies due to their high PVE, small intervals, and large number of initially reported QTLs. In total, 221 candidate genes from critical transcription factor families, such as, bZIP, TPL, GH1, ZF, TIP, OPT, DH19, PPR, PB1, and NB-ARC, were discovered in these M-QTL regions. The findings of the present study reveal key aspects of the genetic mechanisms underlying responses to stress tolerance, as well as agronomical traits including yield. This enhanced understanding significantly advances the potential for improved mapping and marker-assisted selection (MAS) in pea.</p>

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Meta-QTL analysis, candidate gene identification, and validation by GWAS for agronomic traits and stress tolerance in pea (Pisum sativum L.)

  • 期刊论文

  • 发表日期：

    2025年7月24日

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  • Sonam Jha,
  • Zeba Quaiyum,
  • Bishun Deo Prasad,
  • Sangita Sahni,
  • Sahil Singh Mandyal,
  • Ashutosh Singh,
  • Shruti Verma,
  • Rushikesh Sanjay Mane,
  • Teikur Majaw,
  • V. K. Sharma

摘要

In over 95 countries, pea is a vital winter legume crop known for its protein-laden seeds, which are very useful for both dietary and fodder purposes. However, modern agricultural practices and anthropogenic activities leading to climate shift have significantly threatened this valuable crop by increasing the prevalence of abiotic and biotic stresses. To address these challenges, important QTLs and genomic loci are introgressed into modern pea cultivars. We performed a thorough meta-analysis of 594 QTL-related data collected from 17 previous investigations and projected it onto a high-density consensus map based on 6175 markers. Using this method, we identified the most precise genomic areas associated with agronomic characteristics and biotic and abiotic stress tolerances. The study identified 17 M-QTLs with confidence intervals reduced by 4.41-folds as compared to the original QTLs. Genome-wide association (GWA) studies validated the seven M-QTLs (M-QTL1.1, 2.1, 3.1, 4.2, 5.1, 7.1, and 7.3) through their physical positions distributed over six linkage groups, which appeared to be beneficial genomic regions for capitalization by various breeding methodologies due to their high PVE, small intervals, and large number of initially reported QTLs. In total, 221 candidate genes from critical transcription factor families, such as, bZIP, TPL, GH1, ZF, TIP, OPT, DH19, PPR, PB1, and NB-ARC, were discovered in these M-QTL regions. The findings of the present study reveal key aspects of the genetic mechanisms underlying responses to stress tolerance, as well as agronomical traits including yield. This enhanced understanding significantly advances the potential for improved mapping and marker-assisted selection (MAS) in pea.