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<p>The mitochondrial calcium uniporter (MCU), involved in intracellular Ca<sup>2+</sup> signaling and reactive oxygen species (ROS) metabolism, is likely to play a crucial role in plant defense responses. In this study, we identified 11 <i>MCU</i> genes from the genome of <i>Nicotiana tabacum</i> L., and analyzed their phylogenetic relationships, physicochemical properties, structural characteristics, and transcriptional regulation. Additionally, qRT-PCR data were used to analyze the expression profiles of <i>NtMCUs</i> in different tissues, during <i>Ralstonia solanacearum</i> infection, and under exogenous hormone treatment. The results revealed that the 11 <i>NtMCUs</i> were clustered into two subgroups. Subgroups I and II are ancient and stable, but have generated functional differentiation. <i>NtMCU1</i>, <i>NtMCU7</i>, <i>NtMCU11,</i> and <i>NtMCU8</i> exhibited significant differential expression between bacterial wilt-resistant and -susceptible varieties, making them key candidate genes for breeding BW-resistant crops. <i>NtMCUs</i> identification and analysis can contribute to understanding their potential functions in BW defense responses and provide a support for further studies.</p>

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Genome‑wide identification of the MCU gene family and expression analysis during Ralstonia solanacearum infection in Nicotiana tabacum L

  • 期刊论文

  • 发表日期：

    2025年6月7日

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  • Lele Deng,
  • Yangyang Sun,
  • Zhaoyi Wang,
  • Wenwu Yang,
  • Qiao Gao,
  • Ruimei Geng,
  • Zhiliang Xiao,
  • Yanju Shai,
  • Haiying Xiang,
  • Aiguo Yang,
  • Zhiqiang Chen,
  • Xuemei Li,
  • Zhengwen Liu,
  • Min Ren

摘要

The mitochondrial calcium uniporter (MCU), involved in intracellular Ca2+ signaling and reactive oxygen species (ROS) metabolism, is likely to play a crucial role in plant defense responses. In this study, we identified 11 MCU genes from the genome of Nicotiana tabacum L., and analyzed their phylogenetic relationships, physicochemical properties, structural characteristics, and transcriptional regulation. Additionally, qRT-PCR data were used to analyze the expression profiles of NtMCUs in different tissues, during Ralstonia solanacearum infection, and under exogenous hormone treatment. The results revealed that the 11 NtMCUs were clustered into two subgroups. Subgroups I and II are ancient and stable, but have generated functional differentiation. NtMCU1, NtMCU7, NtMCU11, and NtMCU8 exhibited significant differential expression between bacterial wilt-resistant and -susceptible varieties, making them key candidate genes for breeding BW-resistant crops. NtMCUs identification and analysis can contribute to understanding their potential functions in BW defense responses and provide a support for further studies.