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<p>Chloroplasts are vital for plant growth and stress defense due to their roles in photosynthesis and the synthesis of phytohormones like abscisic acid. In this study, we examined a T-DNA insertion mutant, <i>alb7</i>, which exhibits an albino phenotype and is lethal at the seedling stage. Molecular characterization revealed that a T-DNA insertion in the <i>At2g48120</i> locus is tightly associated with the albino phenotype of <i>alb7</i> mutants, and this finding was also supported by the genetic segregation result of <i>alb7</i> heterozygous plants. Additionally, protoplast-based visualization of chloroplast morphology demonstrated that the albino phenotype in <i>alb7</i> homozygous mutants results from severe defects in chloroplast development. Furthermore, gene expression analysis revealed that the <i>ALB7</i> knockout specifically suppresses the transcription of chloroplast genes regulated by Plastid-Encoded RNA polymerase (PEP) but not those regulated by Nuclear-Encoded RNA polymerase (NEP). These findings suggest the crucial role of <i>ALB7</i> in regulating PEP-dependent chloroplast gene transcription, and controlling chloroplast development in <i>Arabidopsis thaliana</i>. Additionally, we found that overexpression of <i>ALB7</i> enhances plant tolerance to drought stress. The improved tolerance appears to be closely linked to abscisic acid, a key stress hormone synthesized in chloroplasts, further supporting the role of <i>ALB7</i> in chloroplast development. </p>

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ALB7 regulates chloroplast development by controlling the PEP-dependent expression of chloroplast genes

  • 期刊论文

  • 发表日期：

    2025年5月17日

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  • Jinhyeong Kim,
  • Geupil Jang

摘要

Chloroplasts are vital for plant growth and stress defense due to their roles in photosynthesis and the synthesis of phytohormones like abscisic acid. In this study, we examined a T-DNA insertion mutant, alb7, which exhibits an albino phenotype and is lethal at the seedling stage. Molecular characterization revealed that a T-DNA insertion in the At2g48120 locus is tightly associated with the albino phenotype of alb7 mutants, and this finding was also supported by the genetic segregation result of alb7 heterozygous plants. Additionally, protoplast-based visualization of chloroplast morphology demonstrated that the albino phenotype in alb7 homozygous mutants results from severe defects in chloroplast development. Furthermore, gene expression analysis revealed that the ALB7 knockout specifically suppresses the transcription of chloroplast genes regulated by Plastid-Encoded RNA polymerase (PEP) but not those regulated by Nuclear-Encoded RNA polymerase (NEP). These findings suggest the crucial role of ALB7 in regulating PEP-dependent chloroplast gene transcription, and controlling chloroplast development in Arabidopsis thaliana. Additionally, we found that overexpression of ALB7 enhances plant tolerance to drought stress. The improved tolerance appears to be closely linked to abscisic acid, a key stress hormone synthesized in chloroplasts, further supporting the role of ALB7 in chloroplast development.