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<p>Soybean is an important food resource for humans and animals and a source of valuable chemicals. As the commercial value of secondary metabolites has increased, genetic engineering of plant-produced valuable compounds has emerged as a promising area for development. In a previous report, syringin (sinapyl alcohol-4-<i>O</i>-glucoside) and coniferin, natural chemical compounds with various pharmacological properties, were produced in transgenic soybeans by overexpressing the chimeric UDP-dependent glucosyltransferases <i>UGT72E3/2</i>. To increase the number of targeted chemicals, the lignin biosynthesis transcriptional activator gene (<i>MYB58)</i> was transformed into soybeans. The resulting transgenic plants were crossed with soybean plants overexpressing the chimeric <i>UGT72E3/2</i> gene. After crossing the β<i>-UGT72E3/2</i> and β<i>-AtMYB58</i> lines, 38 F<sub>1</sub> seeds were harvested from two different combinations of parental crosses. In the polymerase chain reaction (PCR) analysis of transgenes, one plant from β<i>-UGT72E3/2</i> (#7) × β<i>-AtMYB58</i> (#7) contained all transgenes. Their expression was confirmed in the F<sub>1</sub> plants. Subsequent high-performance liquid chromatography (HPLC) analysis on T<sub>2</sub> seeds of β-<i>UGT72E3/2</i> soybean and F<sub>3</sub> seeds from the cross (β-<i>UGT72E3/2</i> × β-<i>AtMYB58</i>) revealed that the average amounts of coniferin and syringin increased by 71% (from 1.50 to 2.57&#xa0;µmol/g dry weight [DW]) and 47% (from 0.34 to 0.50&#xa0;µmol/g DW), respectively. This increase may have resulted from the additional gene expression of <i>AtMYB58.</i></p>

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Gene pyramiding of two genes (UGT72E3/2 and AtMYB58) on the monolignol glucoside pathway increases coniferin and syringin contents in soybean

  • 期刊论文

  • 发表日期：

    2025年4月9日

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  • Jaeyong Han,
  • Hye Jeong Kim,
  • Young-Soo Chung

摘要

Soybean is an important food resource for humans and animals and a source of valuable chemicals. As the commercial value of secondary metabolites has increased, genetic engineering of plant-produced valuable compounds has emerged as a promising area for development. In a previous report, syringin (sinapyl alcohol-4-O-glucoside) and coniferin, natural chemical compounds with various pharmacological properties, were produced in transgenic soybeans by overexpressing the chimeric UDP-dependent glucosyltransferases UGT72E3/2. To increase the number of targeted chemicals, the lignin biosynthesis transcriptional activator gene (MYB58) was transformed into soybeans. The resulting transgenic plants were crossed with soybean plants overexpressing the chimeric UGT72E3/2 gene. After crossing the β-UGT72E3/2 and β-AtMYB58 lines, 38 F1 seeds were harvested from two different combinations of parental crosses. In the polymerase chain reaction (PCR) analysis of transgenes, one plant from β-UGT72E3/2 (#7) × β-AtMYB58 (#7) contained all transgenes. Their expression was confirmed in the F1 plants. Subsequent high-performance liquid chromatography (HPLC) analysis on T2 seeds of β-UGT72E3/2 soybean and F3 seeds from the cross (β-UGT72E3/2 × β-AtMYB58) revealed that the average amounts of coniferin and syringin increased by 71% (from 1.50 to 2.57 µmol/g dry weight [DW]) and 47% (from 0.34 to 0.50 µmol/g DW), respectively. This increase may have resulted from the additional gene expression of AtMYB58.