Scholarlink

<p>The genus <i>Calligonum</i> L. Her (<i>Polygonaceae</i>) comprises 158 species with widespread distribution in regions such as India, China, North Africa, Pakistan, Afghanistan, Saudi Arabia, and South Europe. <i>Calligonum</i> L. is a prominent shrubby psammophyte found in deserts, known for its traditional medicinal uses. The aim of this study is to use Start Codon Target (SCoT) and Inter-Retrotransposon Amplified Polymorphism (IRAP) markers to evaluate the genetic diversity of <i>Calligonum comosum</i> and <i>Calligonum tetrapterum</i> in Saudi Arabia. In addition, it uses the raxmlHPC algorithm and DNA barcoding techniques (matK and rpoC1) to examine phylogenetic relationships. SCoT and IRAP markers revealed distinct genetic patterns, clustering <i>Calligonum</i> species based on their genetic similarities. DNA barcoding of matK and rpoC1 genes provided valuable insights into the evolutionary relationships within <i>Calligonum</i> species. Phylogenetic analyses highlighted well-supported structures with outgroup sequences showing early divergence. Conserved motifs analysis confirmed the presence of shared motifs in both isolated and identified genes, validating the potential use of isolated genes as biomarkers. This comprehensive genetic analysis enhances our understanding of <i>Calligonum</i> species’ genetic relationships, contributing valuable information for taxonomic classification and molecular marker validation. We are the first to add sequence of rpoC1 gene to <i>Calligonum comosum</i> and <i>Calligonum tetrapterum</i> in Gen-Bank. The identified conserved motifs and phylogenetic insights underscore the potential applications of <i>Calligonum</i> genes in various fields, including medicine and biodiversity conservation.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Genetic diversity of closely related Calligounum species collected from Saudi habitats by analyzing the matK and rpoC1 genes, and SCoT and IRAP markers

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年1月13日

查看全文
  • Amal Mohamed AlGarawi,
  • Magda Elsayed Abd-Elgawad

摘要

The genus Calligonum L. Her (Polygonaceae) comprises 158 species with widespread distribution in regions such as India, China, North Africa, Pakistan, Afghanistan, Saudi Arabia, and South Europe. Calligonum L. is a prominent shrubby psammophyte found in deserts, known for its traditional medicinal uses. The aim of this study is to use Start Codon Target (SCoT) and Inter-Retrotransposon Amplified Polymorphism (IRAP) markers to evaluate the genetic diversity of Calligonum comosum and Calligonum tetrapterum in Saudi Arabia. In addition, it uses the raxmlHPC algorithm and DNA barcoding techniques (matK and rpoC1) to examine phylogenetic relationships. SCoT and IRAP markers revealed distinct genetic patterns, clustering Calligonum species based on their genetic similarities. DNA barcoding of matK and rpoC1 genes provided valuable insights into the evolutionary relationships within Calligonum species. Phylogenetic analyses highlighted well-supported structures with outgroup sequences showing early divergence. Conserved motifs analysis confirmed the presence of shared motifs in both isolated and identified genes, validating the potential use of isolated genes as biomarkers. This comprehensive genetic analysis enhances our understanding of Calligonum species’ genetic relationships, contributing valuable information for taxonomic classification and molecular marker validation. We are the first to add sequence of rpoC1 gene to Calligonum comosum and Calligonum tetrapterum in Gen-Bank. The identified conserved motifs and phylogenetic insights underscore the potential applications of Calligonum genes in various fields, including medicine and biodiversity conservation.