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<p>This study innovatively proposes an integrated process of “surfactant-lipase synergistic microwave extraction and in situ cloud point enrichment” to address the issues of cumbersome procedures, high energy consumption, and substantial organic solvent usage associated with the separate steps of traditional sea buckthorn flavonoid extraction and enrichment. This process achieves the simultaneous and efficient completion of flavonoid extraction and concentration. It leverages the synergistic action of surfactant and lipase coupled with microwave assistance to rapidly disrupt sea buckthorn cell walls and promote flavonoid dissolution. Utilizing the cloud point characteristics of surfactants, solvent-free in situ enrichment is achieved through regulation of temperature and salt concentration. Under optimal conditions determined by response surface methodology, the yields of rutin, kaempferol, and isorhamnetin reached 1.305 ± 0.021&#xa0;mg/g, 1.724 ± 0.018&#xa0;mg/g, and 1.269 ± 0.015&#xa0;mg/g, respectively, representing increases of 85%, 76%, and 69% compared to the traditional reflux method. Scanning electron microscopy confirmed the significant cell wall disruption effect of the synergistic system. After optimization, the enrichment rates for rutin, kaempferol, and isorhamnetin attained 93.1 ± 1.66%, 90.8 ± 0.83%, and 91.4 ± 1.89%, respectively. This integrated process combines efficient extraction with solvent-free enrichment, offering operational simplicity and environmental benefits, thereby providing important technical support and theoretical reference for the industrial-scale green production of sea buckthorn flavonoids.</p>

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Integrated Green “Extraction-Enrichment” Process for Sea Buckthorn Berry Flavonoids: Surfactant-Lipase Synergistic Microwave Extraction and In-Situ Cloud Point Enrichment

  • 期刊论文

  • 发表日期：

    2026年3月16日

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  • Jingwei Hao,
  • Xiangkun Zhou,
  • Yi Zhou,
  • Yingying Pei,
  • Yifan Sun,
  • Nan Dong,
  • Chunlei Wan,
  • Dianwei Li

摘要

This study innovatively proposes an integrated process of “surfactant-lipase synergistic microwave extraction and in situ cloud point enrichment” to address the issues of cumbersome procedures, high energy consumption, and substantial organic solvent usage associated with the separate steps of traditional sea buckthorn flavonoid extraction and enrichment. This process achieves the simultaneous and efficient completion of flavonoid extraction and concentration. It leverages the synergistic action of surfactant and lipase coupled with microwave assistance to rapidly disrupt sea buckthorn cell walls and promote flavonoid dissolution. Utilizing the cloud point characteristics of surfactants, solvent-free in situ enrichment is achieved through regulation of temperature and salt concentration. Under optimal conditions determined by response surface methodology, the yields of rutin, kaempferol, and isorhamnetin reached 1.305 ± 0.021 mg/g, 1.724 ± 0.018 mg/g, and 1.269 ± 0.015 mg/g, respectively, representing increases of 85%, 76%, and 69% compared to the traditional reflux method. Scanning electron microscopy confirmed the significant cell wall disruption effect of the synergistic system. After optimization, the enrichment rates for rutin, kaempferol, and isorhamnetin attained 93.1 ± 1.66%, 90.8 ± 0.83%, and 91.4 ± 1.89%, respectively. This integrated process combines efficient extraction with solvent-free enrichment, offering operational simplicity and environmental benefits, thereby providing important technical support and theoretical reference for the industrial-scale green production of sea buckthorn flavonoids.