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<p>Alcoholic liver disease (ALD) is a prevalent metabolic disorder associated with alcohol consumption, posing significant global health challenges. <i>Ecklonia kurome</i>, a marine traditional Chinese medicine (TCM), is recognized as a medicinal resource with hepatoprotective properties. The principal active constituents are polysaccharides, however, the bioavailability of these polysaccharides are poor. In this study, a bidirectional liquid fermentation system was established, and <i>Inonotus hispidus</i> was employed as the fermentation strain to enhance the efficacy of <i>E. kurome</i> in alleviating alcohol-induced liver injury. Compared to prefermentation levels, the concentrations of α-L-guluronic acid and β-D-mannuronic acid increased significantly by 5.86-fold and 5.65-fold, respectively. Following treatment with bidirectional fermentation mycoplasm (SH-KB), biochemical parameters such as triglycerides (TG), alanine aminotransferase (ALT), aspartate aminotransferase (AST), malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), interleukin-6 (IL-6), interleukin-1 beta (IL-1β), and histopathological examinations, showed a significant hepatoprotective effect. The molecular docking revealed that four active monosaccharides exhibited strong binding capabilities with AMP-activated protein kinase (AMPK), peroxisome proliferator-activated receptor alpha (PPAR-α) and sterol regulatory element-binding protein 1 (SREBP-1), which are key regulators of lipid metabolism. And western blotting (WB) results also confirmed this result. All the results indicated that SH-KB significantly mitigated alcohol-induced liver injury through affecting antioxidant enzyme activities, anti-inflammatory properties, and the regulation of lipid metabolism. This study provided meaningful evidence supporting the effect of SH-KB on ALD and offered new directions for efficient utilization of natural oligosaccharide.</p>

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Enhanced Monosaccharide Profile and Anti-Alcoholic Liver Injury Activity in the Liquid Fermentation of Ecklonia kurome by Inonotus hispidus

  • 期刊论文

  • 发表日期：

    2026年5月22日

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  • Baohong Wei,
  • Xue Yang,
  • Jia Liu,
  • Wanghua Bi,
  • Shaoping Wang,
  • Shuman Hu,
  • Xiaoqing Ma,
  • Wenzhe Yang,
  • Changyun Wang,
  • Lin Ju,
  • Huashi Guan

摘要

Alcoholic liver disease (ALD) is a prevalent metabolic disorder associated with alcohol consumption, posing significant global health challenges. Ecklonia kurome, a marine traditional Chinese medicine (TCM), is recognized as a medicinal resource with hepatoprotective properties. The principal active constituents are polysaccharides, however, the bioavailability of these polysaccharides are poor. In this study, a bidirectional liquid fermentation system was established, and Inonotus hispidus was employed as the fermentation strain to enhance the efficacy of E. kurome in alleviating alcohol-induced liver injury. Compared to prefermentation levels, the concentrations of α-L-guluronic acid and β-D-mannuronic acid increased significantly by 5.86-fold and 5.65-fold, respectively. Following treatment with bidirectional fermentation mycoplasm (SH-KB), biochemical parameters such as triglycerides (TG), alanine aminotransferase (ALT), aspartate aminotransferase (AST), malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), interleukin-6 (IL-6), interleukin-1 beta (IL-1β), and histopathological examinations, showed a significant hepatoprotective effect. The molecular docking revealed that four active monosaccharides exhibited strong binding capabilities with AMP-activated protein kinase (AMPK), peroxisome proliferator-activated receptor alpha (PPAR-α) and sterol regulatory element-binding protein 1 (SREBP-1), which are key regulators of lipid metabolism. And western blotting (WB) results also confirmed this result. All the results indicated that SH-KB significantly mitigated alcohol-induced liver injury through affecting antioxidant enzyme activities, anti-inflammatory properties, and the regulation of lipid metabolism. This study provided meaningful evidence supporting the effect of SH-KB on ALD and offered new directions for efficient utilization of natural oligosaccharide.