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<p><i>Isochrysis zhanjiangensis</i> is a dietary microalga renowned for its high content of polyunsaturated fatty acids (PUFAs). However, research on the genes essential for PUFA synthesis in <i>Isochrysis zhanjiangensis</i> is limited. This study successfully isolated two Δ9 fatty acid desaturase genes, <i>IZ-delta9-1</i> and <i>IZ-delta9-2</i>, from <i>Isochrysis zhanjiangensis</i>, which are classified as acyl-lipid desaturases based on phylogenetic analysis. When heterologously expressed in yeast, both genes were confirmed to catalyze the conversion of C16:0 and C18:0 into C16:1 and C18:1, respectively. Furthermore, the impacts of environmental factors on algal growth, fatty acid composition, and transcription levels were explored. Using gas chromatography-mass spectrometry (GC-MS), the fatty acid profiles of <i>I. zhanjiangensis</i> were evaluated. The findings showed that under low temperature (LT) and low nitrogen (LN) conditions, the saturated fatty acids (SFAs) content decreased, and the monounsaturated (MUFAs) and unsaturated fatty acids (UFAs) contents increased. Changes in salinity had a minimal impact on the fatty acid composition. The qPCR analysis revealed that high temperature (HT) and high salt (HS) increased the transcription of <i>IZ-delta9-1</i>, while low nitrogen (LN) and high nitrogen (HN) decreased it. Unlike <i>IZ-delta9-1</i>, the transcription of <i>IZ-delta9-2</i> significantly increased under both low and high temperature treatments, especially in LT groups. Moreover, compared to the control, the transcription levels of <i>IZ-delta9-1</i> decreased under improper salinity and nitrogen concentrations. This study is helpful for understanding the fatty acid synthesis pathway in <i>I. zhanjiangensis</i>.</p>

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Function Characterization and Transcription Analysis of Two Δ9 Fatty Acid Desaturases from Isochrysis zhanjiangensis Under Different Conditions

  • 期刊论文

  • 发表日期：

    2025年5月8日

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  • Xinyue Lu,
  • Lin Zhang,
  • Jichang Han,
  • Jiaojiao Tian,
  • Yumeng Wang,
  • Jiansen Luo,
  • Lingzhi Ye,
  • Suping Hua,
  • Zhen Huang,
  • Jilin Xu

摘要

Isochrysis zhanjiangensis is a dietary microalga renowned for its high content of polyunsaturated fatty acids (PUFAs). However, research on the genes essential for PUFA synthesis in Isochrysis zhanjiangensis is limited. This study successfully isolated two Δ9 fatty acid desaturase genes, IZ-delta9-1 and IZ-delta9-2, from Isochrysis zhanjiangensis, which are classified as acyl-lipid desaturases based on phylogenetic analysis. When heterologously expressed in yeast, both genes were confirmed to catalyze the conversion of C16:0 and C18:0 into C16:1 and C18:1, respectively. Furthermore, the impacts of environmental factors on algal growth, fatty acid composition, and transcription levels were explored. Using gas chromatography-mass spectrometry (GC-MS), the fatty acid profiles of I. zhanjiangensis were evaluated. The findings showed that under low temperature (LT) and low nitrogen (LN) conditions, the saturated fatty acids (SFAs) content decreased, and the monounsaturated (MUFAs) and unsaturated fatty acids (UFAs) contents increased. Changes in salinity had a minimal impact on the fatty acid composition. The qPCR analysis revealed that high temperature (HT) and high salt (HS) increased the transcription of IZ-delta9-1, while low nitrogen (LN) and high nitrogen (HN) decreased it. Unlike IZ-delta9-1, the transcription of IZ-delta9-2 significantly increased under both low and high temperature treatments, especially in LT groups. Moreover, compared to the control, the transcription levels of IZ-delta9-1 decreased under improper salinity and nitrogen concentrations. This study is helpful for understanding the fatty acid synthesis pathway in I. zhanjiangensis.