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<p>Swimming crab (<i>Portunus trituberculatus</i>) eggs contain abundant phospholipids and polyunsaturated fatty acids with specific bioactivities. However, more studies on the functions of other nutrients are needed. Here, a novel sialoglycopeptide from <i>P. trituberculatus</i> eggs, which was termed as <i>Pt</i>-SGP, was isolated with enzymolysis. <i>Pt</i>-SGP was a 6480-Da pure sialoglycopeptide. O-glycan units existed between peptides and sugars. <i>Pt</i>-SGP was composed of 10.69% protein, 71.43% hexose, and 11.27% Neu5Gc acid. Analyzed with HPLC, GC-MS, and NMR spectrometry, the carbohydrate chain of <i>Pt</i>-SGP was {(4GlcNAc1-2Man1-3)/(4GlcNAc1-2Man1-6)Man1-6}/{4Glc1-4}Glc1-4Glc1-3GlcNAc1. The MS spectrometry analysis result showed that the peptide chain was Val-Ala-Thr*-Val-Ser*-Leu-Pro-Arg. The results on mice showed <i>Pt</i>-SGP could reduce body weight gain by 18.13%, white adipose weight by 28.57%, serum TC by 22.32, serum TG by 9.46%, serum LDL-C by 27.69%, hepatic TC by 73.21%, and hepatic TG by 25.20%. Furthernore, it could increase serum HDL-C by 75.96%, mitigate adipocyte size and hepatic lipid accumulation in high fat diet-fed mice. <i>Pt</i>-SGP significantly down-regulated C/EBPα signaling and up-regulated AMPKα signaling in the epididymal fat tissues. The findings indicate that <i>Pt</i>-SGP exhibits notable anti-obesity properties, and can potentially become a promising dietary supplement for anti-obesity.</p>

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Isolation, Characterization and Anti-Obesity Effects of a Novel Sialoglycopeptide Isolated from Portunus trituberculatus Eggs

  • 期刊论文

  • 发表日期：

    2025年5月8日

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  • Shiwei Hu,
  • Shuang Gao,
  • Zhaocai Ren,
  • Peijie Zhang,
  • Jinhui Wang

摘要

Swimming crab (Portunus trituberculatus) eggs contain abundant phospholipids and polyunsaturated fatty acids with specific bioactivities. However, more studies on the functions of other nutrients are needed. Here, a novel sialoglycopeptide from P. trituberculatus eggs, which was termed as Pt-SGP, was isolated with enzymolysis. Pt-SGP was a 6480-Da pure sialoglycopeptide. O-glycan units existed between peptides and sugars. Pt-SGP was composed of 10.69% protein, 71.43% hexose, and 11.27% Neu5Gc acid. Analyzed with HPLC, GC-MS, and NMR spectrometry, the carbohydrate chain of Pt-SGP was {(4GlcNAc1-2Man1-3)/(4GlcNAc1-2Man1-6)Man1-6}/{4Glc1-4}Glc1-4Glc1-3GlcNAc1. The MS spectrometry analysis result showed that the peptide chain was Val-Ala-Thr*-Val-Ser*-Leu-Pro-Arg. The results on mice showed Pt-SGP could reduce body weight gain by 18.13%, white adipose weight by 28.57%, serum TC by 22.32, serum TG by 9.46%, serum LDL-C by 27.69%, hepatic TC by 73.21%, and hepatic TG by 25.20%. Furthernore, it could increase serum HDL-C by 75.96%, mitigate adipocyte size and hepatic lipid accumulation in high fat diet-fed mice. Pt-SGP significantly down-regulated C/EBPα signaling and up-regulated AMPKα signaling in the epididymal fat tissues. The findings indicate that Pt-SGP exhibits notable anti-obesity properties, and can potentially become a promising dietary supplement for anti-obesity.