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<p>Tetraploid oysters frequently exhibit growth and survival rates inferior to diploid and triploid oysters. Tetraploid Pacific oysters (<i>Crassostrea gigas</i>) are pivotal as broodstock in the triploid <i>C. gigas</i> industry. However, the high mortality of tetraploid <i>C. gigas</i> has occurred in production practice with the increasing temperature. The transcriptional patterns of high temperature -susceptible and -tolerant tetraploid <i>C. gigas</i> were compared in response to prolonged thermal treatment. The H was defined as oysters that have not been thermally treated. The susceptible and tolerant oysters after thermal treatment were designed as TS and TH, respectively. The survival rate of tetraploid <i>C. gigas</i> was low (6.33%±2.87%) after thermal treatment. A total of 5147, 5250, and 433 differentially expressed genes (DEGs) were detected in TS <i>vs.</i> TH, TS <i>vs.</i> H, and TH <i>vs.</i> H groups, respectively. Functional enrichment analysis indicated that DEGs were notably enriched in various pathways, including the NF-kappa B signaling pathway, apoptosis, TNF signaling pathway, and arginine and proline metabolism, across both TS <i>vs.</i> TH and TS <i>vs.</i> H groups. Among the DEGs under thermal treatment in susceptible oysters, the translation regulation genes like <i>eIF2a kinase 1</i>, <i>eIF2a kinase 3</i>, <i>MKNK1</i>, and <i>ATF-4</i> exhibited differential expressions. Susceptible oysters displayed a higher number of differentially expressed <i>IAP</i> genes compared to tolerant oysters, while the expression pattern of <i>HSP</i> genes differed between susceptible and tolerant oysters. This study underscores the contrasting response of susceptible and tolerant tetraploid <i>C. gigas</i> to thermal treatment, suggesting that high temperature-susceptible tetraploid <i>C. gigas</i> may be more responsive to rapid alterations in immune response and apoptosis compared to high temperature-tolerant tetraploid <i>C. gigas</i>. It is necessary to improve the high temperature tolerance of tetraploid <i>C. gigas</i> by selective breeding to promote the development of the triploid industry.</p>

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Transcriptome Analysis of the Susceptibility to High Temperature of the Tetraploid Pacific Oyster Crassostrea gigas

  • 期刊论文

  • 发表日期：

    2025年5月8日

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  • Xianchao Bai,
  • Yaru Zhou,
  • Wenlong Bi,
  • Hong Hu,
  • Chengxun Xu,
  • Qi Li

摘要

Tetraploid oysters frequently exhibit growth and survival rates inferior to diploid and triploid oysters. Tetraploid Pacific oysters (Crassostrea gigas) are pivotal as broodstock in the triploid C. gigas industry. However, the high mortality of tetraploid C. gigas has occurred in production practice with the increasing temperature. The transcriptional patterns of high temperature -susceptible and -tolerant tetraploid C. gigas were compared in response to prolonged thermal treatment. The H was defined as oysters that have not been thermally treated. The susceptible and tolerant oysters after thermal treatment were designed as TS and TH, respectively. The survival rate of tetraploid C. gigas was low (6.33%±2.87%) after thermal treatment. A total of 5147, 5250, and 433 differentially expressed genes (DEGs) were detected in TS vs. TH, TS vs. H, and TH vs. H groups, respectively. Functional enrichment analysis indicated that DEGs were notably enriched in various pathways, including the NF-kappa B signaling pathway, apoptosis, TNF signaling pathway, and arginine and proline metabolism, across both TS vs. TH and TS vs. H groups. Among the DEGs under thermal treatment in susceptible oysters, the translation regulation genes like eIF2a kinase 1, eIF2a kinase 3, MKNK1, and ATF-4 exhibited differential expressions. Susceptible oysters displayed a higher number of differentially expressed IAP genes compared to tolerant oysters, while the expression pattern of HSP genes differed between susceptible and tolerant oysters. This study underscores the contrasting response of susceptible and tolerant tetraploid C. gigas to thermal treatment, suggesting that high temperature-susceptible tetraploid C. gigas may be more responsive to rapid alterations in immune response and apoptosis compared to high temperature-tolerant tetraploid C. gigas. It is necessary to improve the high temperature tolerance of tetraploid C. gigas by selective breeding to promote the development of the triploid industry.