Scholarlink

<p>High molecular weight disinfection byproducts (HMW DBPs, MW &gt; 500 Da) significantly contribute to drinking water toxicity yet remain inadequately characterized. This study provides a comprehensive perspective on HMW DBPs through a systematically optimized size exclusion chromatography with diode array detection, fluorescence detection and organic carbon (SEC-DAD-FLD-OCD) approach, enabling a holistic mapping of their multi-dimensional evolution. Results showed chlorination induced oxidative fragmentation of humic substances (HS, 1.2–5 kDa) into building blocks (BB, 0.45–1.2 kDa) and low molecular weight substances (LMWS, &lt; 0.45 kDa). In real drinking water samples, distinct protein-like fluorescence indicated nitrogenous or microbial precursors absent in simulated samples. Parallel factor analysis (PARAFAC) of SEC-DAD chromatograms identified four components: C1 (100–800 Da) correlated with fast-forming LMWS/BB; C2 (800–1200 Da) as BB intermediates; C3 (1.2–4 kDa) and C4 (2–5 kDa) representing persistent HS precursors. Two-dimensional correlation spectroscopy (2D-COS) revealed the relative formation rates followed the order: fulvic-like acids &gt; LMWS &gt; 0 &gt; humic-like acids &gt; BB. Integrated chromatographic peak areas demonstrated rapid HS degradation (52.7% reduction) and LMWS accumulation (117% increase), alongside significant protein-like material accumulation (450%) after 72 h chlorination. This work established SEC-DAD-FLD-OCD as a robust platform for characterizing HMW DBPs, supporting improved water treatment strategies.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Characterization and formation of high molecular weight disinfection byproducts in drinking water: an integrated SEC-DAD-FLD-OCD approach

  • 期刊论文

  • 发表日期：

    2026年4月26日

查看全文
  • Dongxiao Wang,
  • Jiahua Hu,
  • Jiayan Zhao,
  • Feng Zhu,
  • Kai Ye,
  • Qing Zhou,
  • Yang Pan

摘要

High molecular weight disinfection byproducts (HMW DBPs, MW > 500 Da) significantly contribute to drinking water toxicity yet remain inadequately characterized. This study provides a comprehensive perspective on HMW DBPs through a systematically optimized size exclusion chromatography with diode array detection, fluorescence detection and organic carbon (SEC-DAD-FLD-OCD) approach, enabling a holistic mapping of their multi-dimensional evolution. Results showed chlorination induced oxidative fragmentation of humic substances (HS, 1.2–5 kDa) into building blocks (BB, 0.45–1.2 kDa) and low molecular weight substances (LMWS, < 0.45 kDa). In real drinking water samples, distinct protein-like fluorescence indicated nitrogenous or microbial precursors absent in simulated samples. Parallel factor analysis (PARAFAC) of SEC-DAD chromatograms identified four components: C1 (100–800 Da) correlated with fast-forming LMWS/BB; C2 (800–1200 Da) as BB intermediates; C3 (1.2–4 kDa) and C4 (2–5 kDa) representing persistent HS precursors. Two-dimensional correlation spectroscopy (2D-COS) revealed the relative formation rates followed the order: fulvic-like acids > LMWS > 0 > humic-like acids > BB. Integrated chromatographic peak areas demonstrated rapid HS degradation (52.7% reduction) and LMWS accumulation (117% increase), alongside significant protein-like material accumulation (450%) after 72 h chlorination. This work established SEC-DAD-FLD-OCD as a robust platform for characterizing HMW DBPs, supporting improved water treatment strategies.