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<p>Perfluorooctane sulfonate (PFOS) is a dominant per- and polyfluoroalkyl substance (PFAS) at aqueous film-forming foam-impacted sites, yet its specific inhibitory effect on reductive dechlorination of trichloroethene (TCE) remains poorly understood. We investigated TCE dechlorination in a <i>Dehalococcoides</i> (<i>Dhc</i>) and <i>Dehalogenimonas</i> (<i>Dhg</i>)-containing consortium under PFOS stress (20–100 mg/L). While the initial TCE dechlorination rates decreased with increasing PFOS concentrations, the final step of vinyl chloride dechlorination to ethene remained largely unaffected. Microbial community analysis revealed that <i>Dhc</i> and <i>Acetobacterium</i> exhibited greater resilience to 100 mg/L PFOS than <i>Dhg</i> and <i>Clostridium_sensu_stricto_7</i>, with alpha diversity significantly reduced at 100 mg/L PFOS. PFOS, but not perfluorooctanoic acid, was identified as the primary inhibitor of TCE dechlorination. Metagenomic sequencing identified two PFOS-tolerant <i>Dhc</i> strains (THU3 and THU4) that harbored specific reductive dehalogenase genes (<i>vcrA</i> and <i>tceA</i>). Our findings demonstrate the functional resilience of <i>Dhc</i> and its potential as a key agent for bioremediation at PFAS-co-contaminated sites.</p>

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Resilience of Dehalococcoides enables complete reductive dechlorination of trichloroethene to ethene under high PFOS stress

  • 期刊论文

  • 发表日期：

    2026年3月16日

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  • Fang Zhang,
  • Runlei Ge,
  • Chongwen Shi,
  • Tao Long,
  • Kun Yang,
  • Guanghe Li,
  • Lifeng Cao

摘要

Perfluorooctane sulfonate (PFOS) is a dominant per- and polyfluoroalkyl substance (PFAS) at aqueous film-forming foam-impacted sites, yet its specific inhibitory effect on reductive dechlorination of trichloroethene (TCE) remains poorly understood. We investigated TCE dechlorination in a Dehalococcoides (Dhc) and Dehalogenimonas (Dhg)-containing consortium under PFOS stress (20–100 mg/L). While the initial TCE dechlorination rates decreased with increasing PFOS concentrations, the final step of vinyl chloride dechlorination to ethene remained largely unaffected. Microbial community analysis revealed that Dhc and Acetobacterium exhibited greater resilience to 100 mg/L PFOS than Dhg and Clostridium_sensu_stricto_7, with alpha diversity significantly reduced at 100 mg/L PFOS. PFOS, but not perfluorooctanoic acid, was identified as the primary inhibitor of TCE dechlorination. Metagenomic sequencing identified two PFOS-tolerant Dhc strains (THU3 and THU4) that harbored specific reductive dehalogenase genes (vcrA and tceA). Our findings demonstrate the functional resilience of Dhc and its potential as a key agent for bioremediation at PFAS-co-contaminated sites.