Scholarlink

<p>Surfactants are frequently used to enhance the bioremediation of polycyclic aromatic hydrocarbon (PAHs) contaminated soil; however, their complex role in the process of bioremediation has not been fully elucidated. In this study, a PAH-degrading strain, <i>Pseudomonas aeruginosa</i> DH-6, with excellent tolerance to Tween-80 was isolated, and the effect of Tween-80 on the bioremediation of PAHs was re-evaluated using this strain. It was found that Tween-80 not only promoted the solubility of PAHs, but also served as a preferred carbon source, leading to a massive proliferation of DH-6. Moreover, the study demonstrated that an optimal concentration window of Tween-80 maximized PAH biosorption by strain cells. As a result, with the multiple enhancing effects of Tween-80, the dissolved PAHs could be efficiently removed by the combined biosorption–biodegradation process. Whole-genome sequencing and intermediate metabolite analysis revealed that multiple functional genes accounted for the degradation capability of DH-6 for PAHs via salicylic acid and/or phthalic acid pathways. A slurry bioreactor supplemented with spiked Tween-80 achieved 88.1% PAHs removal for the contaminated field soil, significantly reducing remedial time compared with other traditional bioremediation methods. This study highlights the importance of selecting suitable Tween-80 concentration to enhance the remediation of hydrophobic organics in heterogeneous reaction systems such as soil slurry.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

A revisit on the enhancing effect of Tween-80 on the bioremediation of PAH-contaminated soil with a surfactant-compatible strain

  • 期刊论文

  • 发表日期：

    2026年1月1日

查看全文
  • Likun Zhao,
  • Rui Yue,
  • Hua Li,
  • Shiyu Bao,
  • Chaoqi Chen,
  • Kaihong Chen,
  • Jin Qi,
  • Xuhui Mao

摘要

Surfactants are frequently used to enhance the bioremediation of polycyclic aromatic hydrocarbon (PAHs) contaminated soil; however, their complex role in the process of bioremediation has not been fully elucidated. In this study, a PAH-degrading strain, Pseudomonas aeruginosa DH-6, with excellent tolerance to Tween-80 was isolated, and the effect of Tween-80 on the bioremediation of PAHs was re-evaluated using this strain. It was found that Tween-80 not only promoted the solubility of PAHs, but also served as a preferred carbon source, leading to a massive proliferation of DH-6. Moreover, the study demonstrated that an optimal concentration window of Tween-80 maximized PAH biosorption by strain cells. As a result, with the multiple enhancing effects of Tween-80, the dissolved PAHs could be efficiently removed by the combined biosorption–biodegradation process. Whole-genome sequencing and intermediate metabolite analysis revealed that multiple functional genes accounted for the degradation capability of DH-6 for PAHs via salicylic acid and/or phthalic acid pathways. A slurry bioreactor supplemented with spiked Tween-80 achieved 88.1% PAHs removal for the contaminated field soil, significantly reducing remedial time compared with other traditional bioremediation methods. This study highlights the importance of selecting suitable Tween-80 concentration to enhance the remediation of hydrophobic organics in heterogeneous reaction systems such as soil slurry.