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<p>This study used ethanol and acetate as substrates, anaerobic digestion sludge as inoculum, and selected bamboo charcoal with three particle size conditions (&lt; 25, 45–75, 100–250 µm) as the mediating material. While maintaining consistent electrical conductivity levels of bamboo charcoal, we investigated the effect of specific surface area on the chain elongation reactions. Experimental results demonstrated that when bamboo charcoal served as the mediating material, larger specific surface area significantly enhanced the chain elongation for the production of medium-chain fatty acids. Microbial community composition analysis via 16S rRNA sequencing demonstrated that bamboo charcoal intervention promoted the enrichment of <i>Clostridium_sensu_stricto</i>_12, a keystone functional microbe exhibiting statistically significant positive correlations with the yield, electron transfer efficiency, and selectivity of MCFAs. Metagenomic analysis identified the simultaneous presence of reverse <i>β</i>-oxidation and fatty acid biosynthesis pathways, with reverse <i>β</i>-oxidation pathway serving as the dominant route. Notably, the <i>Candidatus_Microthrix</i> genus exhibited dual functionality by engaging in both pathways for MCFAs production.</p>

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Tuning biochar surface area for optimal chain elongation: a strategy for high-efficiency medium-chain fatty acids production

  • 期刊论文

  • 发表日期：

    2026年1月10日

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  • Yuhao Liu,
  • Lei Zhao,
  • Haiping Gu,
  • Xinyi Wang,
  • Bingjie Wang,
  • Kui Wang,
  • Wei Huang,
  • Han Yang,
  • Jingjing Zhang,
  • Junjiao Zhang

摘要

This study used ethanol and acetate as substrates, anaerobic digestion sludge as inoculum, and selected bamboo charcoal with three particle size conditions (< 25, 45–75, 100–250 µm) as the mediating material. While maintaining consistent electrical conductivity levels of bamboo charcoal, we investigated the effect of specific surface area on the chain elongation reactions. Experimental results demonstrated that when bamboo charcoal served as the mediating material, larger specific surface area significantly enhanced the chain elongation for the production of medium-chain fatty acids. Microbial community composition analysis via 16S rRNA sequencing demonstrated that bamboo charcoal intervention promoted the enrichment of Clostridium_sensu_stricto_12, a keystone functional microbe exhibiting statistically significant positive correlations with the yield, electron transfer efficiency, and selectivity of MCFAs. Metagenomic analysis identified the simultaneous presence of reverse β-oxidation and fatty acid biosynthesis pathways, with reverse β-oxidation pathway serving as the dominant route. Notably, the Candidatus_Microthrix genus exhibited dual functionality by engaging in both pathways for MCFAs production.