Scholarlink

<p>Lactic acid bacteria (LAB) are well-studied as probiotics and have multifarious role in promoting human health. They synthesize numerous metabolites which have substantial role in boosting human well-being. Amongst many metabolites synthesized by LAB, exo- oligo/polysaccharides (EOS/EPS), a secondary metabolite, gained substantial importance for its use in food industry as thickeners, bio-emulsifiers or humectants, as micro-carriers in medical field; vast number of EOS/EPS have been studied and characterised which validates their applicability. EOS producing probiotic LAB is desirable for its pertinence in food and health industry. In the current study we have used a probiotic LAB, GC03 which was previously isolated from traditionally fermented wheat preparation. Production of EOS was carried out in synthetic medium and subjected to partial purification. This extracted oligosaccharide was selectively utilised by probiotic <i>Lactiplantibacillus plantarum</i> MCC 2034 and not by enteropathogenic <i>Escherichia coli</i> confirming its prebiotic activity. Also, in comparison to Inulin (a standard prebiotic) GC03 EOS significantly enhances growth of <i>L. plantarum</i> compared to <i>E. coli</i>. Structural elucidation of EOS using <sup>1</sup>H and <sup>13</sup>C NMR spectroscopy revealed an 18-carbon hetero-trisaccharide composed of fructose, glucose and a rare sugar, tagatose in their β-D configuration. EOS could tolerate temperatures up to 219&#xa0;°C, emphasizing its potential for applications in fields requiring high-temperature tolerance.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Structural characterization of prebiotic containing D-tagatose synthesized by lactic acid bacteria

  • 期刊论文

  • 发表日期：

    2026年8月3日

查看全文
  • Nupura Shrikant Joshi,
  • Dattatraya Gopal Naik,
  • Niranjan Prakashrao Patil

摘要

Lactic acid bacteria (LAB) are well-studied as probiotics and have multifarious role in promoting human health. They synthesize numerous metabolites which have substantial role in boosting human well-being. Amongst many metabolites synthesized by LAB, exo- oligo/polysaccharides (EOS/EPS), a secondary metabolite, gained substantial importance for its use in food industry as thickeners, bio-emulsifiers or humectants, as micro-carriers in medical field; vast number of EOS/EPS have been studied and characterised which validates their applicability. EOS producing probiotic LAB is desirable for its pertinence in food and health industry. In the current study we have used a probiotic LAB, GC03 which was previously isolated from traditionally fermented wheat preparation. Production of EOS was carried out in synthetic medium and subjected to partial purification. This extracted oligosaccharide was selectively utilised by probiotic Lactiplantibacillus plantarum MCC 2034 and not by enteropathogenic Escherichia coli confirming its prebiotic activity. Also, in comparison to Inulin (a standard prebiotic) GC03 EOS significantly enhances growth of L. plantarum compared to E. coli. Structural elucidation of EOS using 1H and 13C NMR spectroscopy revealed an 18-carbon hetero-trisaccharide composed of fructose, glucose and a rare sugar, tagatose in their β-D configuration. EOS could tolerate temperatures up to 219 °C, emphasizing its potential for applications in fields requiring high-temperature tolerance.