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<p>Fungal laccases have attracted considerable interest due to their high redox potential, extracellular secretion, and efficient production. This study aimed to evaluate laccase production by <i>Clonostachys</i> (C7, C12, C6-3, C6-4, and B35) and <i>Trichoderma</i> (B1-2, C2, and C7-3) isolates collected from various hosts in Iğdır, Türkiye. Morphological assessment was initially employed for the classification of the isolates, followed by molecular confirmation through ITS- and TEF-1α-based phylogenetic analyses. DNA barcoding identified isolate B1-2 as <i>Trichoderma simmonsii</i>, C2 as <i>Trichoderma afroharzianum</i>, C7-3 as <i>Trichoderma harzianum</i>, while isolates B35, C6-3, C6-4, C7, and C12 were confirmed as <i>Clonostachys rosea</i>. The molecular and phylogenetic analyses consistently supported the taxonomic identification of all isolates. A modified Vogel medium was used to evaluate laccase activity in the <i>Clonostachys</i> and <i>Trichoderma</i> isolates. Among the tested isolates, <i>T. afroharzianum</i> C2 exhibited the highest laccase activity (13.1 U/mL), followed by <i>C. rosea</i> C7 (9.0 U/mL). The Moser kinetic model provided the best fit to the experimental data (R<sup>2</sup> = 0.998). These findings demonstrate the laccase-producing potential of the investigated isolates and highlight their promise as novel fungal resources for biotechnological applications.</p>

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Assessment of laccase activities and identification of Trichoderma and Clonostachys isolates

  • 期刊论文

  • 发表日期：

    2026年7月31日

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  • Tuba Genç Kesimci,
  • Arzu Taşpınar Ünal,
  • Kaan Hürkan,
  • Emine Uygur Göçer

摘要

Fungal laccases have attracted considerable interest due to their high redox potential, extracellular secretion, and efficient production. This study aimed to evaluate laccase production by Clonostachys (C7, C12, C6-3, C6-4, and B35) and Trichoderma (B1-2, C2, and C7-3) isolates collected from various hosts in Iğdır, Türkiye. Morphological assessment was initially employed for the classification of the isolates, followed by molecular confirmation through ITS- and TEF-1α-based phylogenetic analyses. DNA barcoding identified isolate B1-2 as Trichoderma simmonsii, C2 as Trichoderma afroharzianum, C7-3 as Trichoderma harzianum, while isolates B35, C6-3, C6-4, C7, and C12 were confirmed as Clonostachys rosea. The molecular and phylogenetic analyses consistently supported the taxonomic identification of all isolates. A modified Vogel medium was used to evaluate laccase activity in the Clonostachys and Trichoderma isolates. Among the tested isolates, T. afroharzianum C2 exhibited the highest laccase activity (13.1 U/mL), followed by C. rosea C7 (9.0 U/mL). The Moser kinetic model provided the best fit to the experimental data (R2 = 0.998). These findings demonstrate the laccase-producing potential of the investigated isolates and highlight their promise as novel fungal resources for biotechnological applications.