Scholarlink

<p>Shugoshin (SGO) proteins safeguard centromeric cohesion, yet their diversity and potential roles in abiotic stress adaptation remain unexplored in wheat (<i>Triticum aestivum</i>). Here, we performed a genome-wide analysis of the wheat SGO (TaSGO) family, integrating phylogeny, gene structure, promoter cis-elements, subcellular localization prediction, conserved domain enrichment, protein–protein interaction (PPI) mapping, and stress-responsive expression. Phylogenetic reconstruction resolved TaSGOs into two principal clades: a conserved lineage interleaved with <i>Arabidopsis</i> SGOs and a wheat-specific expansion, consistent with duplication and subsequent diversification in the hexaploid genome. Sister pairs within subclades showed near-identical exon–intron architectures, indicating strong purifying constraint on coding regions, whereas UTR length and occasional long introns varied, suggesting regulatory divergence. Promoter scans of 2-kb upstream regions revealed a pervasive light-responsive backbone (G-box/ACE/Box II) overlaid by clade-biased enrichment of stress and hormone motifs (e.g., ABRE, MBS, MeJA), predicting distinct regulatory programs. Localization predictions segregated TaSGOs into a nuclear-dominant cohort, consistent with canonical cohesion roles, and a chloroplast-biased cohort, pointing to possible neofunctionalization. Domain analysis confirmed the defining Shugoshin C-terminal signature and identified sporadic microtubule-associated modules (e.g., TOG/TPR) in a subset of paralogs. STRING-based PPIs revealed a dense TaSGO core with TaSGO9 as a central hub and TaSGO10/TaSGO16 as secondary connectors. Expression profiling under drought, heat, and combined drought and heat showed that both <i>TaSGO1</i> and <i>TaSGO4</i> are inducible, with the strongest and most persistent responses under the combined treatment; <i>TaSGO4</i> consistently exhibited greater and earlier induction (peaking at ~6 h), and tissue atlases indicated root and reproductive enrichment. Collectively, these results delineate a conserved cohesion scaffold with wheat-specific regulatory and cellular stratification, nominate stress-responsive <i>TaSGOs</i> (notably <i>TaSGO4</i>) for functional validation, and provide targets for improving stress resilience in wheat.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Genome-Wide Identification, Motif Architecture, and Stress-Responsive Profiling of Wheat Shugoshins under Heat, Drought, and Dual Stress

  • 期刊论文

  • 发表日期：

    2026年5月26日

查看全文
  • Fatmah Ahmed Safhi,
  • Ahmad M. Alqudah,
  • Hatem E. Semary,
  • Samar G. Thabet

摘要

Shugoshin (SGO) proteins safeguard centromeric cohesion, yet their diversity and potential roles in abiotic stress adaptation remain unexplored in wheat (Triticum aestivum). Here, we performed a genome-wide analysis of the wheat SGO (TaSGO) family, integrating phylogeny, gene structure, promoter cis-elements, subcellular localization prediction, conserved domain enrichment, protein–protein interaction (PPI) mapping, and stress-responsive expression. Phylogenetic reconstruction resolved TaSGOs into two principal clades: a conserved lineage interleaved with Arabidopsis SGOs and a wheat-specific expansion, consistent with duplication and subsequent diversification in the hexaploid genome. Sister pairs within subclades showed near-identical exon–intron architectures, indicating strong purifying constraint on coding regions, whereas UTR length and occasional long introns varied, suggesting regulatory divergence. Promoter scans of 2-kb upstream regions revealed a pervasive light-responsive backbone (G-box/ACE/Box II) overlaid by clade-biased enrichment of stress and hormone motifs (e.g., ABRE, MBS, MeJA), predicting distinct regulatory programs. Localization predictions segregated TaSGOs into a nuclear-dominant cohort, consistent with canonical cohesion roles, and a chloroplast-biased cohort, pointing to possible neofunctionalization. Domain analysis confirmed the defining Shugoshin C-terminal signature and identified sporadic microtubule-associated modules (e.g., TOG/TPR) in a subset of paralogs. STRING-based PPIs revealed a dense TaSGO core with TaSGO9 as a central hub and TaSGO10/TaSGO16 as secondary connectors. Expression profiling under drought, heat, and combined drought and heat showed that both TaSGO1 and TaSGO4 are inducible, with the strongest and most persistent responses under the combined treatment; TaSGO4 consistently exhibited greater and earlier induction (peaking at ~6 h), and tissue atlases indicated root and reproductive enrichment. Collectively, these results delineate a conserved cohesion scaffold with wheat-specific regulatory and cellular stratification, nominate stress-responsive TaSGOs (notably TaSGO4) for functional validation, and provide targets for improving stress resilience in wheat.

Graphical Abstract