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<p>Human and animal health is at risk due to vector-borne diseases. Mosquitoes, mainly <i>Culex quinquefasciatus,</i> play a critical role in the transmission of causative agents of a wide range of diseases such as filariasis, dengue, chikungunya, malaria and several kinds of encephalitis, leading to millions of deaths every year. Chemical based insecticides have been used as the chief control for mosquito management for years. However, the awareness among the people about the detrimental effect of these insecticides requires an eco-friendly approach. The present study was conducted to evaluate the larvicidal potential of <i>Photorhabdus luminescens</i> against the 3rd and 4th instar larvae of <i>C. quinquefasciatus</i> under the laboratory conditions. The rearing of <i>C. quinquefasciatus</i> was done in laboratory and the observed total developmental period ranged between eight to twelve days. A larvicidal bioassay study was conducted using cellular and cell free filtrate that showed dose dependent mortality with time intervals. Results demonstrated that the larvae of <i>Culex</i> mosquitoes were highly vulnerable to <i>P. luminescens</i>. The application of <i>P. luminescens</i> at undiluted cellular filtrate was highly effective against both the 3rd and 4th larval instars of <i>Culex,</i> causing 100% mortality within forty eight hours. The cellular filtrate of <i>P. luminescens</i> caused 82.5% mortality in 3rd instar and 70% mortality in 4th instar larvae at a treatment concentration of 8 × 10<sup>3</sup>&#xa0;CFU/mL. Mortality enhanced considerably with concentration and exposure period. Probit analysis showed lower LC<sub>50</sub> values for 3rd instar larvae, signifying higher vulnerability, and Tukey’s HSD confirmed considerable variation among treatments. It can be suggested from the present investigation that <i>P. luminescens</i> can be applied for the effective management of <i>Culex</i> mosquitoes. Further investigations are necessary to evaluate the usefulness of this entomopathogenic bacterium at the natural breeding sites of mosquitoes.</p>

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A study on larvicidal potential of Photorhabdus luminescens against Culex quinquefasciatus

  • 期刊论文

  • 发表日期：

    2026年5月8日

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  • Yankita Negi,
  • Preety Tomar,
  • Sakshi Sharma,
  • Neelam Yadav,
  • Rajeshwari Negi,
  • Ashok Yadav,
  • Ajar Nath Yadav

摘要

Human and animal health is at risk due to vector-borne diseases. Mosquitoes, mainly Culex quinquefasciatus, play a critical role in the transmission of causative agents of a wide range of diseases such as filariasis, dengue, chikungunya, malaria and several kinds of encephalitis, leading to millions of deaths every year. Chemical based insecticides have been used as the chief control for mosquito management for years. However, the awareness among the people about the detrimental effect of these insecticides requires an eco-friendly approach. The present study was conducted to evaluate the larvicidal potential of Photorhabdus luminescens against the 3rd and 4th instar larvae of C. quinquefasciatus under the laboratory conditions. The rearing of C. quinquefasciatus was done in laboratory and the observed total developmental period ranged between eight to twelve days. A larvicidal bioassay study was conducted using cellular and cell free filtrate that showed dose dependent mortality with time intervals. Results demonstrated that the larvae of Culex mosquitoes were highly vulnerable to P. luminescens. The application of P. luminescens at undiluted cellular filtrate was highly effective against both the 3rd and 4th larval instars of Culex, causing 100% mortality within forty eight hours. The cellular filtrate of P. luminescens caused 82.5% mortality in 3rd instar and 70% mortality in 4th instar larvae at a treatment concentration of 8 × 103 CFU/mL. Mortality enhanced considerably with concentration and exposure period. Probit analysis showed lower LC50 values for 3rd instar larvae, signifying higher vulnerability, and Tukey’s HSD confirmed considerable variation among treatments. It can be suggested from the present investigation that P. luminescens can be applied for the effective management of Culex mosquitoes. Further investigations are necessary to evaluate the usefulness of this entomopathogenic bacterium at the natural breeding sites of mosquitoes.