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<p>The study is aimed at optimizing the production of a multi-enzyme cocktail by <i>Bacillus cereus</i> MCCB 101 using soyabean meal as a sole fermentation substrate for potential aquafeed supplementation. Initial screening revealed enhanced bacterial growth and digestive enzyme activities in soyabean meal-based medium compared to ZoBell’s medium, producing 40.56 U/mL amylase, 36.84 U/mL cellulase, 9.72 U/mL pectinase, 26.58 U/mL mannanase, 11.04 U/mL chitinase, 12.24 U/mL protease, 73.14 U/mL xylanase, and 13.62 U/mL tannase. The enzyme cocktail was relatively stable at room temperature (25&#xa0;°C) and 4&#xa0;°C for one month. Process parameters were optimized using a One Factor at a Time (OFAT) approach followed by Response Surface Methodology (RSM), which identified 44&#xa0;°C, pH 7.5, 25 ppt salinity, 3.5% substrate concentration, as the optimal conditions. Under these conditions, an average of 1.4-fold increase in enzyme activities was achieved. These findings demonstrate the feasibility of soyabean meal-based enzyme production for sustainable aquafeed applications.</p>

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Enhanced production of enzyme cocktail by Bacillus cereus MCCB 101 using response surface methodology for aquafeed supplementation

  • 期刊论文

  • 发表日期：

    2026年4月30日

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  • Limmy Thazhunga Pailykutty,
  • Akshay Puthumanakudy Sajeev,
  • Riya George,
  • Gopika Venu Sreebindu,
  • Valsamma Joseph,
  • Issac Sarojini Bright Singh

摘要

The study is aimed at optimizing the production of a multi-enzyme cocktail by Bacillus cereus MCCB 101 using soyabean meal as a sole fermentation substrate for potential aquafeed supplementation. Initial screening revealed enhanced bacterial growth and digestive enzyme activities in soyabean meal-based medium compared to ZoBell’s medium, producing 40.56 U/mL amylase, 36.84 U/mL cellulase, 9.72 U/mL pectinase, 26.58 U/mL mannanase, 11.04 U/mL chitinase, 12.24 U/mL protease, 73.14 U/mL xylanase, and 13.62 U/mL tannase. The enzyme cocktail was relatively stable at room temperature (25 °C) and 4 °C for one month. Process parameters were optimized using a One Factor at a Time (OFAT) approach followed by Response Surface Methodology (RSM), which identified 44 °C, pH 7.5, 25 ppt salinity, 3.5% substrate concentration, as the optimal conditions. Under these conditions, an average of 1.4-fold increase in enzyme activities was achieved. These findings demonstrate the feasibility of soyabean meal-based enzyme production for sustainable aquafeed applications.