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<p>The accumulation of synthetic polymeric waste is a significant environmental hazard since it is poisonous and non-biodegradable. PHAs and other microbially generated biopolymers offer an environmentally friendly alternative due to their biodegradability and biocompatibility. A novel PHA-producing bacterium identified by 16S rRNA sequencing as <i>Priestia flexa</i> JP1 (NCBI accession no. PP989347.2) was isolated from oil-contaminated soil used in this investigation. Statistical analysis using one-way ANOVA followed by Tukey’s post-hoc test revealed that the specific growth rate in medium M3 was significantly higher than in M1 and M2 (<i>p</i> ≤ 0.05). All the experiments were performed in triplicate (n = 3). Maximum dry cell biomass was obtained in M3 (0.565&#xa0;g/L), while the lowest in M2 (0.335&#xa0;g/L). JP1 outperformed previously reported results for similar strains with a maximum PHA output of 0.5&#xa0;g/L under optimal growth conditions, which corresponds to 82.5% of dry cell weight in medium M3. The polymer was confirmed by Fourier Transform Infrared Spectroscopy and characterized for morphology by Scanning Electron Microscopy, and to study the thermal properties of the extracted polymer, Differential Scanning Calorimetry was performed. These findings highlight <i>P. flexa</i> JP1 as a promising candidate for high-yield, sustainable PHA production, with potential application in replacing conventional plastics. While DSC melting range suggests predominantly PHA composition, definitive monomer identification requires <sup>13</sup>C NMR spectroscopy, which will be pursued in future investigations. Further optimization of biomass accumulation and downstream purification, alongside the integration of cost-effective substrates, could enhance its scalability for industrial use.</p>

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Production and characterization of polyhydroxyalkanoates by a novel strain Priestia flexa JP1

  • 期刊论文

  • 发表日期：

    2026年4月21日

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  • Rakhi Pandey,
  • Garima Mathur

摘要

The accumulation of synthetic polymeric waste is a significant environmental hazard since it is poisonous and non-biodegradable. PHAs and other microbially generated biopolymers offer an environmentally friendly alternative due to their biodegradability and biocompatibility. A novel PHA-producing bacterium identified by 16S rRNA sequencing as Priestia flexa JP1 (NCBI accession no. PP989347.2) was isolated from oil-contaminated soil used in this investigation. Statistical analysis using one-way ANOVA followed by Tukey’s post-hoc test revealed that the specific growth rate in medium M3 was significantly higher than in M1 and M2 (p ≤ 0.05). All the experiments were performed in triplicate (n = 3). Maximum dry cell biomass was obtained in M3 (0.565 g/L), while the lowest in M2 (0.335 g/L). JP1 outperformed previously reported results for similar strains with a maximum PHA output of 0.5 g/L under optimal growth conditions, which corresponds to 82.5% of dry cell weight in medium M3. The polymer was confirmed by Fourier Transform Infrared Spectroscopy and characterized for morphology by Scanning Electron Microscopy, and to study the thermal properties of the extracted polymer, Differential Scanning Calorimetry was performed. These findings highlight P. flexa JP1 as a promising candidate for high-yield, sustainable PHA production, with potential application in replacing conventional plastics. While DSC melting range suggests predominantly PHA composition, definitive monomer identification requires 13C NMR spectroscopy, which will be pursued in future investigations. Further optimization of biomass accumulation and downstream purification, alongside the integration of cost-effective substrates, could enhance its scalability for industrial use.