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<p>Honey bees are crucial pollinators for global agriculture, but their survival is threatened by multiple factors, including viral diseases that contribute significantly to colony collapse disorder. The objective of this study was to characterize honey bees and identify viruses in samples collected from apiaries and crop fields in the Junín region of Peru. For molecular analysis and virus identification, small RNA libraries were generated and sequenced. The presence of viruses was subsequently validated by RT-PCR. Both morphometric and molecular analyses confirmed that the honey bees in the provinces studied in Junín belong to <i>A. mellifera ligustica</i> (lineage C). Sequencing results revealed the presence of <i>Apis mellifera</i> filamentous virus (AmFV) and Deformed wing virus (DWV-A) in apiaries in all provinces under study. The incidence of AmFV varied between 43 and 76%, while that of DWV-A ranged between 34 and 64%. The province of Satipo recorded the lowest incidence (43% for AmFV and 34% for DWV-A), while Chupaca had the highest (76% for AmFV and 64% for DWV-A). The isolated AmFV showed 97.40% identity to the clade isolated from Switzerland and Hungary, forming a clade close to that of New Zealand. The DWV-A isolate showed 99.17% identity to that of Spain and New Zealand but was grouped as a separate clade. This is the first report in Peru of AmFV and DWV-A, and our findings underscore the need to implement health management and control strategies in honeybee populations in the Junín region to safeguard food security and the sustainability of local beekeeping.</p>

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First report of Apis mellifera filamentous virus (AmFV) and Deformed wing virus (DWV-A) in Peruvian honey bees

  • 期刊论文

  • 发表日期：

    2026年3月21日

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  • Gabriela Angela Carrillo-Ordóñez,
  • Nancy Párraga-Melgarejo,
  • Renán Angelo García-Acevedo,
  • Xiomara Junchaya-Huamán,
  • Francisco Correa,
  • Emerson Clovis Carrasco-Lozano

摘要

Honey bees are crucial pollinators for global agriculture, but their survival is threatened by multiple factors, including viral diseases that contribute significantly to colony collapse disorder. The objective of this study was to characterize honey bees and identify viruses in samples collected from apiaries and crop fields in the Junín region of Peru. For molecular analysis and virus identification, small RNA libraries were generated and sequenced. The presence of viruses was subsequently validated by RT-PCR. Both morphometric and molecular analyses confirmed that the honey bees in the provinces studied in Junín belong to A. mellifera ligustica (lineage C). Sequencing results revealed the presence of Apis mellifera filamentous virus (AmFV) and Deformed wing virus (DWV-A) in apiaries in all provinces under study. The incidence of AmFV varied between 43 and 76%, while that of DWV-A ranged between 34 and 64%. The province of Satipo recorded the lowest incidence (43% for AmFV and 34% for DWV-A), while Chupaca had the highest (76% for AmFV and 64% for DWV-A). The isolated AmFV showed 97.40% identity to the clade isolated from Switzerland and Hungary, forming a clade close to that of New Zealand. The DWV-A isolate showed 99.17% identity to that of Spain and New Zealand but was grouped as a separate clade. This is the first report in Peru of AmFV and DWV-A, and our findings underscore the need to implement health management and control strategies in honeybee populations in the Junín region to safeguard food security and the sustainability of local beekeeping.