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<p>Significant advancements have been made in the management and prevention of inflammation over the past few decades. The current study aims to investigate the inflammatory potential of the mucin extracted from the land slug <i>Sarasinula plebeia</i> against oxidative stress and inflammation-related processes. Thirty-six distinct peaks were found by GC–MS analysis of this mucin. Lecithin, n-Hexadecanoic acid, 5-Hydroxymethylfurfural, oleic acid ethyl ester, 9,12-Octadecadienoic acid, methyl ester, and lastly, hyoleic safflower oil, are the main peaks. The mucin reacts more selectively toward COX-1 with IC<sub>50</sub> 22.17 ± 0.63 µg ml<sup>−1</sup> compared to Celecoxib IC<sub>50</sub> 21.27 ± 0.604 µg ml <sup>−1</sup>, but it exhibited a lower activity towards COX-2 with IC<sub>50</sub> 7.905 ± 0.32 µg ml<sup>−1</sup> as compared to Celecoxib IC<sub>50</sub> 2.59 ± 0.126 µg ml<sup>−1</sup>. In this model, neither slug gel nor celecoxib altered systemic oxidative‐stress biomarkers (serum SOD, MDA). Biochemical analysis showed that the higher dose of slug gel reduced serum&#xa0;prostaglandin E2 (PGE2) and tumor necrosis factor-alpha (TNF-α) levels. The present results indicated that both 250 mg and 500 mg concentrations of the slug preparation topical gel significantly reduced rat paw edema, demonstrating an anti-inflammatory effect similar to that of celecoxib 250 mg gel. This potential was revealed by the paw edema diameter test and the measurement of anti-inflammatory markers, PGE2 and TNF-α. In conclusion, <i>S. plebeia</i> mucin has high bioactive capacities and that its crude extract exhibited a good range of anti-inflammatory action, making it valuable for the pharmaceutical business.</p>

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GC/MS metabolites profiling of Sarasinula plebeia slug mucin in correlation to its anti-inflammatory activity targeting, Cyclooxygenase 2 via in-vitro and in-vivo approach

  • 期刊论文

  • 发表日期：

    2026年3月4日

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  • Amina M. Ibrahim,
  • Inas Y. Younis,
  • Nabil M. Selim,
  • Ahmed M. Fayez,
  • Baher Daihom,
  • Mostafa Y. Morad,
  • Engy Mohsen

摘要

Significant advancements have been made in the management and prevention of inflammation over the past few decades. The current study aims to investigate the inflammatory potential of the mucin extracted from the land slug Sarasinula plebeia against oxidative stress and inflammation-related processes. Thirty-six distinct peaks were found by GC–MS analysis of this mucin. Lecithin, n-Hexadecanoic acid, 5-Hydroxymethylfurfural, oleic acid ethyl ester, 9,12-Octadecadienoic acid, methyl ester, and lastly, hyoleic safflower oil, are the main peaks. The mucin reacts more selectively toward COX-1 with IC50 22.17 ± 0.63 µg ml−1 compared to Celecoxib IC50 21.27 ± 0.604 µg ml −1, but it exhibited a lower activity towards COX-2 with IC50 7.905 ± 0.32 µg ml−1 as compared to Celecoxib IC50 2.59 ± 0.126 µg ml−1. In this model, neither slug gel nor celecoxib altered systemic oxidative‐stress biomarkers (serum SOD, MDA). Biochemical analysis showed that the higher dose of slug gel reduced serum prostaglandin E2 (PGE2) and tumor necrosis factor-alpha (TNF-α) levels. The present results indicated that both 250 mg and 500 mg concentrations of the slug preparation topical gel significantly reduced rat paw edema, demonstrating an anti-inflammatory effect similar to that of celecoxib 250 mg gel. This potential was revealed by the paw edema diameter test and the measurement of anti-inflammatory markers, PGE2 and TNF-α. In conclusion, S. plebeia mucin has high bioactive capacities and that its crude extract exhibited a good range of anti-inflammatory action, making it valuable for the pharmaceutical business.