Incorporation of in-vitro, in-vivo, and in-silico antidiabetic activity of a bioactive compound extracted from the red macroalgae Gracilaria corticata
摘要
Marine algae have been identified as valuable resources because of their nutritional composition and possible biological applications. The antidiabetic effects of an ethanol extract of the red macroalgae Gracilaria corticata were investigated. The phytochemical characteristics of a G. corticata ethanol extract (GCE) were examined. UV–vis spectroscopy and FTIR analysis were performed on the ethanol extract. The inhibition of alpha-amylase and alpha-glucosidase, as well as in vivo experiments utilizing zebrafish, were used to evaluate the antidiabetic effectiveness in vitro. The highest binding energy was observed for α-glucosidase, with a docking score of -1.83 kcal mol−1, and for α-amylase with a score of -1.95 kcal mol−1, both of which were from in-silico studies in which the binding affinities were tested. With a docking score of -3.98 kcal mol−1, nonanoic acid has the lowest binding energy toward α-amylase. The docking score for dodecanamide was the lowest, at -4.49 kcal mol−1. Both the α-amylase inhibitory and α-glucosidase activities showed greater promise in the GCE. Our results provide more evidence of the pharmacological, potential of GCE and suggest that bioactive chemicals from this species could serve as lead molecules in the development of effective diabetes treatments.
Graphical Abstract