Morpho-phylogenic, pathogenic characterization and biocontrol of Fusarium species infecting cucurbits in Algeria
摘要
Cucurbits are one of the most important vegetable crops in the world. This crop is exposed to several pathogenic species of the genus Fusarium, which cause very significant yield losses. Sampling concerns the Ziban region (Biskra, Algeria). Fusarium spp. were isolated from characteristic symptoms, morphologically characterized, molecular identified using tef1 and ITS primers and seedling pathogenicity tested. In addition, the biocontrol potential of two Trichoderma species “T. harzianum and T. virens” were evaluated against the studied pathogen. 25 Fusarium spp. were isolated and identified, revealing 16% F. solani and 4% F. falciforme, both belonging to the F. solani complex, 20% F. oxysporum, 28% F. equiseti, 12% F. nygamai, 8% F. prolifératum and 4% F. brachygibbosum. These seven identified Fusarium species are reported for the first time as Fusarium pathogens of cucurbits in Algeria. Molecular identification results confirmed the morphological characterization. Seedling pathogenicity assay showed that 36% of the Fusarium isolates inhibited germination by more than 53%. The three Trichoderma strains showed high biocontrol potential against Fusarium with inhibition of more than 70%. These results showed that Trichoderma species are promising biocontrol solutions against the studied Fusarium species which is a significant problem in the region.