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<p>Medicinal mushrooms have long garnered attention for their nutritional value and health benefits, in part, because of their abundant polysaccharides. Three medicinal mushrooms, <i>Trametes versicolor</i>, <i>Hericium erinaceus</i>, and <i>Ganoderma subresinosum,</i> are particularly common in Asia and known to contain these valuable compounds; however, their transcriptomes as well as the genes responsible for polysaccharide biosynthesis are unknown. In this study, we used the Illumina system to elucidate the <i>de novo</i> transcriptomes of these mushrooms. A total of 22,583 unigenes for <i>T. versicolor</i>, 22,304 for <i>H. erinaceus</i>, and 27,282 for <i>G. subresinosum</i> were assembled. Of these, approximately 70%–80% of the unigenes from each data set were functionally annotated using multiple databases. Between 159 and 181 unigenes involved in polysaccharide biosynthesis and metabolism pathways were identified, and 14 key genes essential for the biosynthesis of monosaccharides were present in these species. The transcriptomic data of <i>T. versicolor</i> revealed, for the first time, the gene encoding dTDP-4-dehydrorhamnose reductase. A similarity comparison of enzymes involved in the polysaccharide synthesis pathway exhibited a close relationship between the enzymes of <i>T. versicolor</i> and <i>G. subresinosum</i>, whereas those of <i>H. erinaceus</i> belonged to a distantly related group. Quantitative reverse transcription polymerase chain reaction validated the expression of selected genes identified by RNA sequencing. These results represent the first comprehensive transcriptome data for <i>T. versicolor</i>, <i>H. erinaceus</i>, and <i>G. subresinosum</i>, which provide insight into the polysaccharide biosynthesis pathway and may lead to their improved production through biotechnology techniques.</p>

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De novo transcriptome analyses of medicinal mushrooms reveal potential genes in polysaccharide biosynthesis

  • 期刊论文

  • 发表日期：

    2026年1月13日

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  • Le Bich Hang Pham,
  • Hai Quynh Do,
  • Van Giap Nguyen,
  • Duy Trinh Nguyen,
  • Long Tran,
  • Thi Minh Hien Pham,
  • Minh Cuc Luu,
  • Hai Ha Nguyen,
  • Thu Ha Tran,
  • Thi Thu Hien Le

摘要

Medicinal mushrooms have long garnered attention for their nutritional value and health benefits, in part, because of their abundant polysaccharides. Three medicinal mushrooms, Trametes versicolor, Hericium erinaceus, and Ganoderma subresinosum, are particularly common in Asia and known to contain these valuable compounds; however, their transcriptomes as well as the genes responsible for polysaccharide biosynthesis are unknown. In this study, we used the Illumina system to elucidate the de novo transcriptomes of these mushrooms. A total of 22,583 unigenes for T. versicolor, 22,304 for H. erinaceus, and 27,282 for G. subresinosum were assembled. Of these, approximately 70%–80% of the unigenes from each data set were functionally annotated using multiple databases. Between 159 and 181 unigenes involved in polysaccharide biosynthesis and metabolism pathways were identified, and 14 key genes essential for the biosynthesis of monosaccharides were present in these species. The transcriptomic data of T. versicolor revealed, for the first time, the gene encoding dTDP-4-dehydrorhamnose reductase. A similarity comparison of enzymes involved in the polysaccharide synthesis pathway exhibited a close relationship between the enzymes of T. versicolor and G. subresinosum, whereas those of H. erinaceus belonged to a distantly related group. Quantitative reverse transcription polymerase chain reaction validated the expression of selected genes identified by RNA sequencing. These results represent the first comprehensive transcriptome data for T. versicolor, H. erinaceus, and G. subresinosum, which provide insight into the polysaccharide biosynthesis pathway and may lead to their improved production through biotechnology techniques.