Scholarlink

<p>Endophytic fungi represent promising sources of bioactive secondary metabolites with novel therapeutic potential. In this study, two endophytic fungi isolated from the medicinal plant&#xa0;<i>Plectranthus amboinicus</i>&#xa0;were identified as&#xa0;<i>Cladosporium xanthochromaticum</i>&#xa0;and&#xa0;<i>C. tenuissimum</i>&#xa0;based on 18S rRNA gene sequence analysis. Gas chromatography-mass spectrometry (GC–MS) analysis of ethyl acetate crude fungal extracellular extracts (FE) revealed oleic acid as the predominant compound in&#xa0;<i>C. xanthochromaticum</i>, while 1-nonadecene and 1-hexadecane were major constituents in&#xa0;<i>C. tenuissimum</i>. Both fungal extracts demonstrated potent antimicrobial activity against&#xa0;<i>Escherichia coli</i>,&#xa0;<i>Staphylococcus aureus</i>,&#xa0;<i>Pseudomonas aeruginosa</i>,&#xa0;<i>Bacillus subtilis</i>, and&#xa0;<i>Candida albicans</i>&#xa0;in disc diffusion assays. Minimum inhibitory concentration (MIC) values ranged from 3.12–6.25&#xa0;mg/mL against bacterial pathogens and 12.5–50&#xa0;mg/mL against&#xa0;<i>C. albicans</i>. Notably,&#xa0;<i>C. tenuissimum</i>&#xa0;FE exhibited significant antibiofilm activity, achieving 84.45% inhibition against&#xa0;<i>S. aureus</i>&#xa0;and 62% inhibition against&#xa0;<i>P. aeruginosa</i>&#xa0;at ½ × MIC concentrations. Additionally,&#xa0;<i>C. xanthochromaticum</i>&#xa0;displayed considerable antioxidant activity with a half maximal inhibitory concentration (IC₅₀) value of 301.12&#xa0;µg/mL. This study represents the first comprehensive report of antimicrobial, antifungal, and antibiofilm properties of&#xa0;<i>C. xanthochromaticum</i>. These findings provide novel insights into the therapeutic potential of&#xa0;<i>Cladosporium</i>&#xa0;species and warrant further investigation of their bioactive compounds and mechanisms of action for pharmaceutical applications.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Endophytic Cladosporium species from Plectranthus amboinicus as potential sources of antimicrobial and antioxidant agents

  • 期刊论文

  • 发表日期：

    2026年1月15日

查看全文
  • Chaithanya Gowda Anantha Narasimha Murthy,
  • Shivani Gopalacalony Mahesh,
  • Anjali Menon Girish,
  • Prerana Rajendra Yadav,
  • Varshini Kiran Gowda,
  • Deeshma Kandothum Purath

摘要

Endophytic fungi represent promising sources of bioactive secondary metabolites with novel therapeutic potential. In this study, two endophytic fungi isolated from the medicinal plant Plectranthus amboinicus were identified as Cladosporium xanthochromaticum and C. tenuissimum based on 18S rRNA gene sequence analysis. Gas chromatography-mass spectrometry (GC–MS) analysis of ethyl acetate crude fungal extracellular extracts (FE) revealed oleic acid as the predominant compound in C. xanthochromaticum, while 1-nonadecene and 1-hexadecane were major constituents in C. tenuissimum. Both fungal extracts demonstrated potent antimicrobial activity against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, and Candida albicans in disc diffusion assays. Minimum inhibitory concentration (MIC) values ranged from 3.12–6.25 mg/mL against bacterial pathogens and 12.5–50 mg/mL against C. albicans. Notably, C. tenuissimum FE exhibited significant antibiofilm activity, achieving 84.45% inhibition against S. aureus and 62% inhibition against P. aeruginosa at ½ × MIC concentrations. Additionally, C. xanthochromaticum displayed considerable antioxidant activity with a half maximal inhibitory concentration (IC₅₀) value of 301.12 µg/mL. This study represents the first comprehensive report of antimicrobial, antifungal, and antibiofilm properties of C. xanthochromaticum. These findings provide novel insights into the therapeutic potential of Cladosporium species and warrant further investigation of their bioactive compounds and mechanisms of action for pharmaceutical applications.