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<p>Lactic acid bacteria (LAB) were isolated from various fermented sources, including milk, curd, palm wine, and lassi, to identify potential probiotic candidates. A total of 80 samples were collected and initially analysed using conventional microbiological techniques, such as Gram staining, catalase testing, endospore formation, and motility assays. After cultivation on de Man, Rogosa, and Sharpe (MRS) agar, 35 isolates were identified as LAB based on their colony morphology and biochemical characteristics. These isolates underwent a series of probiotic assessments, including their ability to withstand low pH, bile, sodium chloride (NaCl), phenol, and pancreatin enzyme, simulating gastrointestinal conditions. Of the 35 isolates, 16 strains passed these rigorous evaluations, with strain 2018 showing remarkable potential. Further analysis of strain 2018, classified as <i>Enterococcus faecium</i>, revealed it to be gram-positive, cocci-shaped, non-motile, non-endospore-forming, and catalase-negative. It demonstrated strong survival capabilities under simulated gastrointestinal conditions, with notable auto-aggregation and hydrophobicity against apolar hydrocarbons. Safety evaluations confirmed its non-hemolytic and Deoxyribonuclease (DNase) negative characteristics. Importantly, strain 2018 exhibited the highest antioxidant and anti-inflammatory activities among the isolates. These findings highlight the potential of LAB strains, particularly strain 2018, for human health applications, given their robust probiotic properties, safety profiles, and bioactive compound production.</p>

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Screening and characterization of Enterococcus faecium as a potential probiotic: probiotic parameters and efficacy evaluation

  • 期刊论文

  • 发表日期：

    2026年1月23日

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  • Padmaja Phani Nidamarthi,
  • Senthamil Selvi Poongavanam,
  • Abraham Peele Karlapudi

摘要

Lactic acid bacteria (LAB) were isolated from various fermented sources, including milk, curd, palm wine, and lassi, to identify potential probiotic candidates. A total of 80 samples were collected and initially analysed using conventional microbiological techniques, such as Gram staining, catalase testing, endospore formation, and motility assays. After cultivation on de Man, Rogosa, and Sharpe (MRS) agar, 35 isolates were identified as LAB based on their colony morphology and biochemical characteristics. These isolates underwent a series of probiotic assessments, including their ability to withstand low pH, bile, sodium chloride (NaCl), phenol, and pancreatin enzyme, simulating gastrointestinal conditions. Of the 35 isolates, 16 strains passed these rigorous evaluations, with strain 2018 showing remarkable potential. Further analysis of strain 2018, classified as Enterococcus faecium, revealed it to be gram-positive, cocci-shaped, non-motile, non-endospore-forming, and catalase-negative. It demonstrated strong survival capabilities under simulated gastrointestinal conditions, with notable auto-aggregation and hydrophobicity against apolar hydrocarbons. Safety evaluations confirmed its non-hemolytic and Deoxyribonuclease (DNase) negative characteristics. Importantly, strain 2018 exhibited the highest antioxidant and anti-inflammatory activities among the isolates. These findings highlight the potential of LAB strains, particularly strain 2018, for human health applications, given their robust probiotic properties, safety profiles, and bioactive compound production.