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<p>This study investigates the antibacterial and antibiofilm potential of quercetin against carbapenem and cephalosporin-resistant clinical <i>Escherichia coli</i> and evaluating its antioxidant activity. <i>E.coli</i>- EI the most antibiotic-resistant strain was further employed in evaluating the antibacterial and antibiofilm activity of quercetin. <i>E.coli</i>-EI preformed biofilm-biomass was eliminated by 53.1% when treated with quercetin (200&#xa0;mg/mL). Additionally, SEM micrographs of treated biofilms showed elimination of most of the biofilm polysaccharide-matrix, decreased cell density, and cell membrane disruption of some bacterial cells. Furthermore, PCR and RT-qPCR results revealed downregulation of <i>adrA</i> biofilm gene. DPPH assay results indicated that quercetin dose-dependent antioxidant activity with IC₅₀ value of 53.06&#xa0;mg/mL. Moreover, molecular docking studies revealed that quercetin is able to form several stabilizing interactions like hydrogen bonds and hydrophobic interactions, with AdrA-protein, displaying effective binding-capacity of -6.2&#xa0;kcal/mol. Consequently, quercetin represents a natural feasible compound for treating <i>E.coli</i> infections in addition to its antioxidant competence.</p>

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Quercetin-mediated repression of adrA gene expression in Escherichia coli: dual roles in antibiofilm activity and oxidative stress regulation

  • 期刊论文

  • 发表日期：

    2025年10月2日

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  • Alzhraa Ali Mohamed,
  • Eslam Abdelhakim Seyam,
  • Sameh A. Hussein,
  • Mohamed Abdel‐Haleem

摘要

This study investigates the antibacterial and antibiofilm potential of quercetin against carbapenem and cephalosporin-resistant clinical Escherichia coli and evaluating its antioxidant activity. E.coli- EI the most antibiotic-resistant strain was further employed in evaluating the antibacterial and antibiofilm activity of quercetin. E.coli-EI preformed biofilm-biomass was eliminated by 53.1% when treated with quercetin (200 mg/mL). Additionally, SEM micrographs of treated biofilms showed elimination of most of the biofilm polysaccharide-matrix, decreased cell density, and cell membrane disruption of some bacterial cells. Furthermore, PCR and RT-qPCR results revealed downregulation of adrA biofilm gene. DPPH assay results indicated that quercetin dose-dependent antioxidant activity with IC₅₀ value of 53.06 mg/mL. Moreover, molecular docking studies revealed that quercetin is able to form several stabilizing interactions like hydrogen bonds and hydrophobic interactions, with AdrA-protein, displaying effective binding-capacity of -6.2 kcal/mol. Consequently, quercetin represents a natural feasible compound for treating E.coli infections in addition to its antioxidant competence.