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<p>Recently, the application of organophosphate pesticides has been restricted due to their environmental and health risks; however, certain pesticides, such as ethoprophos, continue to be employed particularly for the control of root-knot nematodes in greenhouse vegetable production. In this study, we reported the isolation and characterization of bacterial strains capable of tolerating and degrading ethoprophos from greenhouse soils with a prolonged history of pesticide exposure. Following soil treatment with ethoprophos over several weeks to enrich tolerant bacterial populations, six distinct bacterial species were isolated from both treated and untreated soil cultures. Species identification was performed through 16S rRNA gene sequencing, and the corresponding accession numbers have been deposited in public nucleotide databases. The ethoprophos-tolerant strains identified belong to the genera <i>Achromobacter</i>, <i>Acinetobacter</i>, <i>Pseudomonas</i> and <i>Stenotrophomonas</i>, which are underrepresented in the Literature regarding ethoprophos tolerance and degradation. Biochemical profiling was performed, and minimum inhibitory concentration assays revealed that these four isolates exhibited tolerance to ethoprophos on agar plates at concentrations ranging from 8&#xa0;g/L to 18&#xa0;g/L. The bacteria were also tolerant to other organophosphates including glyphosate and chlorpyrifos and to different pesticide groups including carbamates and pyrethroids. Notably, <i>Pseudomonas</i> sp. and <i>Achromobacter</i> sp. demonstrated ethoprophos degradation efficiencies of 32% and 36%, respectively. These findings highlight novel bacterial candidates with significant potential for use in bioremediation strategies and environmental biomonitoring of organophosphate-contaminated ecosystems. This study lays the groundwork for future investigations into the molecular mechanisms of pesticide tolerance and degradation and provides a valuable microbial resource for sustainable environmental management practices.</p>

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Isolation and characterization of soil bacteria exhibiting broad pesticide tolerance and ethoprophos biodegradation ability

  • 期刊论文

  • 发表日期：

    2025年9月17日

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  • Evrim Elçin

摘要

Recently, the application of organophosphate pesticides has been restricted due to their environmental and health risks; however, certain pesticides, such as ethoprophos, continue to be employed particularly for the control of root-knot nematodes in greenhouse vegetable production. In this study, we reported the isolation and characterization of bacterial strains capable of tolerating and degrading ethoprophos from greenhouse soils with a prolonged history of pesticide exposure. Following soil treatment with ethoprophos over several weeks to enrich tolerant bacterial populations, six distinct bacterial species were isolated from both treated and untreated soil cultures. Species identification was performed through 16S rRNA gene sequencing, and the corresponding accession numbers have been deposited in public nucleotide databases. The ethoprophos-tolerant strains identified belong to the genera Achromobacter, Acinetobacter, Pseudomonas and Stenotrophomonas, which are underrepresented in the Literature regarding ethoprophos tolerance and degradation. Biochemical profiling was performed, and minimum inhibitory concentration assays revealed that these four isolates exhibited tolerance to ethoprophos on agar plates at concentrations ranging from 8 g/L to 18 g/L. The bacteria were also tolerant to other organophosphates including glyphosate and chlorpyrifos and to different pesticide groups including carbamates and pyrethroids. Notably, Pseudomonas sp. and Achromobacter sp. demonstrated ethoprophos degradation efficiencies of 32% and 36%, respectively. These findings highlight novel bacterial candidates with significant potential for use in bioremediation strategies and environmental biomonitoring of organophosphate-contaminated ecosystems. This study lays the groundwork for future investigations into the molecular mechanisms of pesticide tolerance and degradation and provides a valuable microbial resource for sustainable environmental management practices.