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<p>The dimorphic fungus <i>Sporothrix globosa</i> (<i>S. globosa</i>) can induce both localized and disseminated infections, posing a considerable threat to the health of animal and human. The morphological shift between the yeast and mycelial phases is crucial for its virulence. However, the transcriptional networks implicated in its dimorphic transition remain incompletely elucidated. This study aims to explore the pathogenesis of sporotrichosis caused by <i>S. globosa</i> through transcriptomics analysis. This study investigated <i>S. globosa</i> mycelial and yeast samples through microscopic study. Total ribonucleic acid (RNA) was extracted to construct RNA transcriptome libraries for whole transcriptome sequencing. Differentially expressed genes (DEGs) between the <i>S. globosa</i> mycelial and yeast samples were identified and subjected to Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Protein Families (Pfam) enrichment analyses. Yeast samples showed stronger fluorescence patterns compared to mycelial samples under the microscope. Transcriptomic analysis indicated significant gene upregulation occurs during the dimorphic transition of <i>S. globosa</i> from the mycelial to the yeast phase. Some upregulated DEGs were associated with chitin and mitogen-activated protein kinase (MAPK) signaling pathway, while downregulated DEGs predominantly enriched in the metabolic pathways. Our results offer key insights into the dimorphism and pathogenesis of <i>S. globosa</i> and provide a basis for the development of novel antifungal agents.</p>

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Novel targets and signaling pathway into the mechanisms of Sporothrix globosa dimorphism from transcriptomics data

  • 期刊论文

  • 发表日期：

    2025年9月22日

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  • Ziwenyan Zhou,
  • Qiang Fu,
  • Lei Tang,
  • Fujun Huang,
  • Junhong Wang,
  • Yuanjiao Lei,
  • Mengya Huang

摘要

The dimorphic fungus Sporothrix globosa (S. globosa) can induce both localized and disseminated infections, posing a considerable threat to the health of animal and human. The morphological shift between the yeast and mycelial phases is crucial for its virulence. However, the transcriptional networks implicated in its dimorphic transition remain incompletely elucidated. This study aims to explore the pathogenesis of sporotrichosis caused by S. globosa through transcriptomics analysis. This study investigated S. globosa mycelial and yeast samples through microscopic study. Total ribonucleic acid (RNA) was extracted to construct RNA transcriptome libraries for whole transcriptome sequencing. Differentially expressed genes (DEGs) between the S. globosa mycelial and yeast samples were identified and subjected to Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Protein Families (Pfam) enrichment analyses. Yeast samples showed stronger fluorescence patterns compared to mycelial samples under the microscope. Transcriptomic analysis indicated significant gene upregulation occurs during the dimorphic transition of S. globosa from the mycelial to the yeast phase. Some upregulated DEGs were associated with chitin and mitogen-activated protein kinase (MAPK) signaling pathway, while downregulated DEGs predominantly enriched in the metabolic pathways. Our results offer key insights into the dimorphism and pathogenesis of S. globosa and provide a basis for the development of novel antifungal agents.