Scholarlink

<p>The false anemonefish <i>Amphiprion ocellaris</i> Cuvier, 1830 characterized by its magnificent colors and patterns, provides excellent material to study the cellular and genomic bases of color diversity in vertebrates. For genetic engineering, such as the utilization of CRISPR-Cas9 technologies, <i>A. ocellaris</i> embryos can be removed from their substrate at an early stage of development for ease of microinjection, but are no longer in care of their parents. Thus, requiring the artificial incubation of embryos without parental care yieds in lower survival and hatching rates. In this study, we introduced novel modifications to an artificial incubator design with the aim of improving the survival and hatching rate of anemonefish embryos without parental care, hoping to minimize the negative influence of environmental factors on pre- and post-hatching fish larvae. Next, we investigated the impact of environmental parameters, including temperature, salinity, and water circulation, on the survival rate, hatching rate, incubation period, and larval fitness of <i>A</i>. <i>ocellaris</i> embryos raised in artificial incubators. Hatching rates differed significantly across different temperatures and salinities and water circulation, with the highest hatching rate recorded at 29&#xa0;°C and 32 ppt salinity and flow rate of 8000 L/hour, respectively. For both temperature and salinity, incubation period was significantly longer at the lowest conditions (25&#xa0;°C, 25 ppt). Low temperature and low salinity produced longer larvae at hatching. Our new artificial incubator design improved the survival rate of anemonefish embryos without parental care by 1.7 times compared to previous artificial systems, and thus is an important step forward in the utilization of this model species in developmental studies.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Improvement of artificial incubators and studying the effects of environmental factors on anemonefish Amphiprion ocellaris embryo’s development

  • 期刊论文

  • 发表日期：

    2025年7月9日

查看全文
  • Hai-Thanh Thi Nguyen,
  • Olivia Bosc,
  • Sac Duy Nguyen

摘要

The false anemonefish Amphiprion ocellaris Cuvier, 1830 characterized by its magnificent colors and patterns, provides excellent material to study the cellular and genomic bases of color diversity in vertebrates. For genetic engineering, such as the utilization of CRISPR-Cas9 technologies, A. ocellaris embryos can be removed from their substrate at an early stage of development for ease of microinjection, but are no longer in care of their parents. Thus, requiring the artificial incubation of embryos without parental care yieds in lower survival and hatching rates. In this study, we introduced novel modifications to an artificial incubator design with the aim of improving the survival and hatching rate of anemonefish embryos without parental care, hoping to minimize the negative influence of environmental factors on pre- and post-hatching fish larvae. Next, we investigated the impact of environmental parameters, including temperature, salinity, and water circulation, on the survival rate, hatching rate, incubation period, and larval fitness of A. ocellaris embryos raised in artificial incubators. Hatching rates differed significantly across different temperatures and salinities and water circulation, with the highest hatching rate recorded at 29 °C and 32 ppt salinity and flow rate of 8000 L/hour, respectively. For both temperature and salinity, incubation period was significantly longer at the lowest conditions (25 °C, 25 ppt). Low temperature and low salinity produced longer larvae at hatching. Our new artificial incubator design improved the survival rate of anemonefish embryos without parental care by 1.7 times compared to previous artificial systems, and thus is an important step forward in the utilization of this model species in developmental studies.