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<p>One of the most harmful and prevalent microbiological infections that can affect anyone of any age or gender is urinary tract infection (UTI). Antibiotic resistance of <i>Enterobacterales</i> poses a major challenge in the treatment of UTI. In low- and middle-income countries (LMICs), standard microbiological methods (i.e. urine culture and simple disk diffusion test) are considered the “gold standard” for bacterial identification and drug susceptibility testing, but 16sr RNA and DNA sequencing are less commonly used. This study aimed to evaluate the prevalence of UTIs and antibiotic resistance profiles through urine culture by16S rRNA. A total of 68 samples were collected from different patients. Pathogens from urine samples were identified using culture and biochemical test. Kirby-Bauer's disc diffusion method was used for antibiotic susceptibility testing. Molecular identification was double-checked through 16Sr RNA and DNA sequencing method. Results showed that; out of 68 samples, 39 samples were positive cultures, 11 (28%) were male and 28 (71%) female, with an average age of 35.5 years. The most predominant organisms were <i>Escherichia</i> spp. (38.13%) followed by <i>Klebsiella</i> spp. (27.86%), <i>Pseudomonas aeruginosa</i> (16.23%), and <i>Staphylococcus aureus</i> (11.50%). The most antibiotic-resistant isolates were from <i>Escherichia</i> spp. and <i>Klebsiella</i> spp. and the four isolates that showed high resistance rate were identified using the 16S rRNA technique. According to the BLAST analysis, isolate 1, 2, 3 and 4 showed a 100% similarity to <i>Escherichia coli</i>, <i>Escherichia fergusonii</i><i>, </i><i>Klebsiella pneumonia</i> and <i>Klebsiella variicola</i>, respectively. Uropathogenic identification using 16S rRNA technique could be a useful tool for diagnosing the UTI in clinical samples in combination with the conventional culture.</p>

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Isolation and molecular analysis of multi-drug resistant bacteria causing urinary tract infections using 16S rRNA technique: a study on urine samples

  • 期刊论文

  • 发表日期：

    2025年7月3日

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  • Eman M. Handak,
  • Heba I. Elkhouly

摘要

One of the most harmful and prevalent microbiological infections that can affect anyone of any age or gender is urinary tract infection (UTI). Antibiotic resistance of Enterobacterales poses a major challenge in the treatment of UTI. In low- and middle-income countries (LMICs), standard microbiological methods (i.e. urine culture and simple disk diffusion test) are considered the “gold standard” for bacterial identification and drug susceptibility testing, but 16sr RNA and DNA sequencing are less commonly used. This study aimed to evaluate the prevalence of UTIs and antibiotic resistance profiles through urine culture by16S rRNA. A total of 68 samples were collected from different patients. Pathogens from urine samples were identified using culture and biochemical test. Kirby-Bauer's disc diffusion method was used for antibiotic susceptibility testing. Molecular identification was double-checked through 16Sr RNA and DNA sequencing method. Results showed that; out of 68 samples, 39 samples were positive cultures, 11 (28%) were male and 28 (71%) female, with an average age of 35.5 years. The most predominant organisms were Escherichia spp. (38.13%) followed by Klebsiella spp. (27.86%), Pseudomonas aeruginosa (16.23%), and Staphylococcus aureus (11.50%). The most antibiotic-resistant isolates were from Escherichia spp. and Klebsiella spp. and the four isolates that showed high resistance rate were identified using the 16S rRNA technique. According to the BLAST analysis, isolate 1, 2, 3 and 4 showed a 100% similarity to Escherichia coli, Escherichia fergusonii, Klebsiella pneumonia and Klebsiella variicola, respectively. Uropathogenic identification using 16S rRNA technique could be a useful tool for diagnosing the UTI in clinical samples in combination with the conventional culture.