Characterization of azo dye-degrading Tistrella mobilis isolated from industrial effluents: a molecular perspective
摘要
Industrial waste, particularly from the textile and leather industries, is a major contributor to environmental pollution through the release of chemical effluents and dyes. Despite legislative efforts, managing this pollution remains a significant challenge, especially in regions like Delhi-NCR, India. This study aims to identify and characterize microorganisms with dye-degrading potential from industrial wastewater, employing both biochemical and molecular techniques. Eleven bacterial isolates were initially identified, six of which demonstrated over 50% decolorization of Sudan Orange dye. Among these, isolate B03 exhibited the highest degradation efficiency, achieving 70% decolorization. Optimization of azoreductase enzyme activity revealed optimal performance at pH 8 and 45 °C, with minimal impact from varying carbon and nitrogen sources. Kinetic analysis of the enzyme yielded a Km of 12.939 mM, a Vmax of 20.408 U/ml, a Kcat of 28.10 s⁻1, and a Kcat/Km of 2.17 mM⁻1 s⁻1, indicating moderate substrate affinity. HPLC and SDS-PAGE analyses confirmed the identity and molecular weight (29 kDa) of the enzyme. Further, 16S rRNA sequencing identified the strain as Tistrella mobilis MSVN.1, with phylogenetic analysis placing it within the Tistrella genus. This study advances the understanding of biological dye degradation and paves the way for the development of sustainable bioremediation strategies in industrial wastewater management.