Scholarlink

<p>Pectinases, a group of enzymes that degrade pectin, are crucial in various industrial applications. This study aimed to isolate, characterize, and evaluate the multifunctional traits of pectinase-producing bacteria from partially decayed fruits and soil in Sidi Bel Abbes, Algeria. Out of 80 isolates screened on pectinase screening agar medium (PSAM), five <i>Bacillus</i> species demonstrated significant pectinase activity, with pectin degradation indices (PDI) of 6.9–7.9 and enzyme yields of 7.9–10 U/mL under submerged fermentation. Morphological, biochemical, and 16S rRNA gene analyses identified these strains as <i>Bacillus amyloliquefaciens</i> SL9, <i>B. licheniformis</i> SC3, <i>B. mojavensis</i> ST1, <i>B. velezensis</i> SPC3, and <i>B. siamensis</i> SL6. Besides pectinase production, these <i>Bacillus</i> spp. displayed plant growth-promoting traits (including nitrogen fixation, phosphate solubilization, siderophore production, and indole-3-acetic acid (IAA) synthesis) and antimicrobial activity against several phytopathogens. They also produced diverse extracellular enzymes and demonstrated tolerance to salinity, pH variation, and high temperatures underscoring their adaptability to challenging environments. These findings highlight the potential of these <i>Bacillus</i> spp. for industrial enzyme production, biocontrol, and sustainable agricultural applications, particularly in stress-prone conditions.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Revealing of multifunctional newly isolated Bacillus strains from Algerian wastes with high pectinase activity for sustainable biotechnological application

  • 期刊论文

  • 发表日期：

    2025年2月8日

查看全文
  • Fatima Zohra Kaissar,
  • Mohamed Lamine Benine,
  • Bouziane Abbouni,
  • Sid Ahmed Saadi,
  • Giovanni Emiliani,
  • Sara Barberini,
  • Seif El Islam Lebouachera

摘要

Pectinases, a group of enzymes that degrade pectin, are crucial in various industrial applications. This study aimed to isolate, characterize, and evaluate the multifunctional traits of pectinase-producing bacteria from partially decayed fruits and soil in Sidi Bel Abbes, Algeria. Out of 80 isolates screened on pectinase screening agar medium (PSAM), five Bacillus species demonstrated significant pectinase activity, with pectin degradation indices (PDI) of 6.9–7.9 and enzyme yields of 7.9–10 U/mL under submerged fermentation. Morphological, biochemical, and 16S rRNA gene analyses identified these strains as Bacillus amyloliquefaciens SL9, B. licheniformis SC3, B. mojavensis ST1, B. velezensis SPC3, and B. siamensis SL6. Besides pectinase production, these Bacillus spp. displayed plant growth-promoting traits (including nitrogen fixation, phosphate solubilization, siderophore production, and indole-3-acetic acid (IAA) synthesis) and antimicrobial activity against several phytopathogens. They also produced diverse extracellular enzymes and demonstrated tolerance to salinity, pH variation, and high temperatures underscoring their adaptability to challenging environments. These findings highlight the potential of these Bacillus spp. for industrial enzyme production, biocontrol, and sustainable agricultural applications, particularly in stress-prone conditions.

Graphical Abstract