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<p><i>Salvia miltiorrhiza</i> Bunge, a perennial herb of the Lamiaceae, is renowned for its principal bioactive ingredient, tanshinone, which possesses substantial pharmacological value in the treatment of cardiovascular and cerebrovascular diseases. Studies have shown that exogenous elicitor treatments can elevate tanshinone accumulation in <i>S. miltiorrhiza</i>; however, most of these investigations have focused on the transcriptional control of target genes by specific transcription factors. However, the relationship between endogenous phytohormones and tanshinone biosynthesis remains poorly understood. In this study, <i>S. miltiorrhiza</i> seedlings were treated with 200&#xa0;mg L⁻¹ yeast extract (YE) and the accumulation of tanshinones. Concurrently, the contents of indole-3-acetic acid (IAA) and gibberellin (GA) were significantly up-regulattion, while the content of abscisic acid (ABA) was significantly decreased. <i>TAR2</i> is a key gene in IAA biosynthesis, and its expression is significantly up-regulated in <i>S. miltiorrhiza</i> following YE treatment. Consequently, hairy root lines overexpressing <i>SmTAR2</i> were established (designated TAR-OE lines). High-performance liquid chromatography (HPLC) analysis revealed that the tanshinone content in <i>SmTAR2</i> overexpression lines was significantly higher than that in the wild-type lines. The results indicated that YE optimally promoted tanshinones accumulation by up-regulating the expression of <i>SmTAR2</i>. In summary, this work not only defined a more appropriate YE concentration for eliciting tanshinone biosynthesis in <i>S. miltiorrhiza</i>, but also identified the auxin-synthesis gene <i>SmTAR2</i> as a potent enhancer of tanshinone accumulation.</p>

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Yeast extract modulates the auxin biosynthesis pathway via TAR2 to enhance tanshinone accumulation in salvia miltiorrhiza

  • 期刊论文

  • 发表日期：

    2026年7月2日

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  • Xuhui Huang,
  • Xiyuan Sun,
  • Qiuyan Leng,
  • Xiao Feng,
  • Jinlin Xie,
  • Xuanfeng Du,
  • Xuexue Deng,
  • Yuanyuan Jiang,
  • Ruiwu Yang,
  • Yunsong Zhang,
  • Li Zhang

摘要

Salvia miltiorrhiza Bunge, a perennial herb of the Lamiaceae, is renowned for its principal bioactive ingredient, tanshinone, which possesses substantial pharmacological value in the treatment of cardiovascular and cerebrovascular diseases. Studies have shown that exogenous elicitor treatments can elevate tanshinone accumulation in S. miltiorrhiza; however, most of these investigations have focused on the transcriptional control of target genes by specific transcription factors. However, the relationship between endogenous phytohormones and tanshinone biosynthesis remains poorly understood. In this study, S. miltiorrhiza seedlings were treated with 200 mg L⁻¹ yeast extract (YE) and the accumulation of tanshinones. Concurrently, the contents of indole-3-acetic acid (IAA) and gibberellin (GA) were significantly up-regulattion, while the content of abscisic acid (ABA) was significantly decreased. TAR2 is a key gene in IAA biosynthesis, and its expression is significantly up-regulated in S. miltiorrhiza following YE treatment. Consequently, hairy root lines overexpressing SmTAR2 were established (designated TAR-OE lines). High-performance liquid chromatography (HPLC) analysis revealed that the tanshinone content in SmTAR2 overexpression lines was significantly higher than that in the wild-type lines. The results indicated that YE optimally promoted tanshinones accumulation by up-regulating the expression of SmTAR2. In summary, this work not only defined a more appropriate YE concentration for eliciting tanshinone biosynthesis in S. miltiorrhiza, but also identified the auxin-synthesis gene SmTAR2 as a potent enhancer of tanshinone accumulation.