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<p><i>Syneilesis aconitifolia</i> (Bunge) Maxim. is a medicinal food homologous plant with multiple pharmacological properties. Flavonoids are the primary active constituents of <i>S. aconitifolia</i>. Despite the extensive number of chemical and pharmacological studies conducted on the flavonoids of <i>S. aconitifolia</i>, transcriptomic and genomic information for this species is extremely limited. To address this knowledge gap, we sequenced the transcriptomes of the leaves, stems, roots, and rhizomes of <i>S. aconitifolia</i> using the DNBSEQ platform. A total of 191,541 unigenes were obtained, 160,534 of which were annotated in at least one of the seven public databases investigated. High-performance liquid chromatography was employed to detect the contents of daidzin, isoquercitrin, and quercitrin in the different tissues of <i>S</i>. <i>aconitifolia</i>, with the highest concentrations of these substances being found in the leaves. Correlation analysis between gene expression levels and the contents of the three flavonoids indicated that six unigenes were strongly and positively correlated with isoquercitrin and quercitrin contents. The functional characteristics of flavonoid 3’-hydroxylases were investigated using bioinformatics and phylogenetic analysis. A total of 23 transcription factors were found to participate in the regulation of flavonoid biosynthesis, 18 of which were identified as members of the MYB family. In this work, we identified the genes encoding enzymes that regulate the biosynthesis of flavonoids in <i>S. aconitifolia</i>. This study represents the first report on the transcriptome of <i>S. aconitifolia</i> and lays a foundation for subsequent research on flavonoid biosynthesis in this and related species.</p>

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Comparative transcriptome analysis provides new insights into the flavonoid biosynthesis pathway in Syneilesis aconitifolia

  • 期刊论文

  • 发表日期：

    2026年5月11日

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  • Jingjing Zhang,
  • Wanying Xie,
  • Tingyu Shan,
  • Jingyao Xu,
  • Xinxin Zhong,
  • Zhiwei Wang,
  • Jinshibo Fan,
  • Jiawen Wu,
  • Yijia Tao

摘要

Syneilesis aconitifolia (Bunge) Maxim. is a medicinal food homologous plant with multiple pharmacological properties. Flavonoids are the primary active constituents of S. aconitifolia. Despite the extensive number of chemical and pharmacological studies conducted on the flavonoids of S. aconitifolia, transcriptomic and genomic information for this species is extremely limited. To address this knowledge gap, we sequenced the transcriptomes of the leaves, stems, roots, and rhizomes of S. aconitifolia using the DNBSEQ platform. A total of 191,541 unigenes were obtained, 160,534 of which were annotated in at least one of the seven public databases investigated. High-performance liquid chromatography was employed to detect the contents of daidzin, isoquercitrin, and quercitrin in the different tissues of S. aconitifolia, with the highest concentrations of these substances being found in the leaves. Correlation analysis between gene expression levels and the contents of the three flavonoids indicated that six unigenes were strongly and positively correlated with isoquercitrin and quercitrin contents. The functional characteristics of flavonoid 3’-hydroxylases were investigated using bioinformatics and phylogenetic analysis. A total of 23 transcription factors were found to participate in the regulation of flavonoid biosynthesis, 18 of which were identified as members of the MYB family. In this work, we identified the genes encoding enzymes that regulate the biosynthesis of flavonoids in S. aconitifolia. This study represents the first report on the transcriptome of S. aconitifolia and lays a foundation for subsequent research on flavonoid biosynthesis in this and related species.