<p>Unfavorable temperatures during germination can significantly disrupt the physiological and biochemical processes crucial for seed germination, posing a challenge to soybean crop establishment and overall yield potential. Seed priming has emerged as a technique that has the potential to enhance crop establishment under high-temperature stress conditions. The presented study evaluated the impact of seed priming [without seed priming, hydropriming, and osmopriming with polyethylene glycol (PEG) 6000 @ −0.5, −1.0, −1.5 and −2.0 megapascal (MPa) for 6&#xa0;h] at different temperatures (25, 30, 35, and 40℃) on physiological and biochemical parameters under controlled conditions. The experiment was conducted twice in factorial complete randomized design, each replicated four times. The results showed that seed priming with PEG @ −1.5&#xa0;MPa recorded higher speed of germination, germination percentage, total seedling length, seedling dry weight, and vigor indices at 30&#xa0;°C than other seed priming treatments and temperatures. Seed treated with PEG @ −1.5&#xa0;MPa recorded higher speed of germination (15.3 and 8.2%), seedling vigor index I and II (19.6% and 13.0%, and 10.3% and 6.5%) over control and hydropriming, respectively. Compared to other priming treatments, PEG @ −1.5&#xa0;MPa primed seeds exhibited significantly lower electrical conductivity, higher dehydrogenase activity, and higher percentage of stained seeds at the different temperatures tested (25, 30, 35, and 40℃). This implies that seed priming with PEG @ −1.5&#xa0;MPa could serve as a valuable method to enhance the physiological and biochemical parameters of soybean at high temperatures, potentially fostering early plant development and augmenting yield potential.</p>

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Physiological and biochemical responses to seed priming with polyethylene glycol under variable temperatures in soybean

  • Simranpreet Singh Bola,
  • Harpreet Kaur Virk,
  • Navjyot Kaur

摘要

Unfavorable temperatures during germination can significantly disrupt the physiological and biochemical processes crucial for seed germination, posing a challenge to soybean crop establishment and overall yield potential. Seed priming has emerged as a technique that has the potential to enhance crop establishment under high-temperature stress conditions. The presented study evaluated the impact of seed priming [without seed priming, hydropriming, and osmopriming with polyethylene glycol (PEG) 6000 @ −0.5, −1.0, −1.5 and −2.0 megapascal (MPa) for 6 h] at different temperatures (25, 30, 35, and 40℃) on physiological and biochemical parameters under controlled conditions. The experiment was conducted twice in factorial complete randomized design, each replicated four times. The results showed that seed priming with PEG @ −1.5 MPa recorded higher speed of germination, germination percentage, total seedling length, seedling dry weight, and vigor indices at 30 °C than other seed priming treatments and temperatures. Seed treated with PEG @ −1.5 MPa recorded higher speed of germination (15.3 and 8.2%), seedling vigor index I and II (19.6% and 13.0%, and 10.3% and 6.5%) over control and hydropriming, respectively. Compared to other priming treatments, PEG @ −1.5 MPa primed seeds exhibited significantly lower electrical conductivity, higher dehydrogenase activity, and higher percentage of stained seeds at the different temperatures tested (25, 30, 35, and 40℃). This implies that seed priming with PEG @ −1.5 MPa could serve as a valuable method to enhance the physiological and biochemical parameters of soybean at high temperatures, potentially fostering early plant development and augmenting yield potential.