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Objective <p>To investigate the regulatory effect of moxibustion on rats with Crohn disease (CD) and explore the relationship between this effect and alterations in colonic histone H3 lysine 27 acetylation (H3K27ac).</p> Methods <p>Ten out of 36 Sprague-Dawley rats were randomly selected as the control group using a random number table method. The remaining 26 rats received an enema of 2,4,6-trinitrobenzene sulfonic acid to establish a CD model. Successfully modeled rats were then randomly divided into a model group, an herb-insulated moxibustion group, and a sulfasalazine group, with 8 rats in each group. The herb-insulated moxibustion group received herb-insulated moxibustion at Qihai (CV6) and bilateral Tianshu (ST25), and the sulfasalazine group received sulfasalazine via gavage. Histopathological changes in the colonic tissue were assessed using hematoxylin-eosin staining. The serum levels of inflammatory factors [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] were measured using the enzyme-linked immunosorbent assay. The protein expression of colonic H3K27ac was detected using Western blotting and immunohistochemistry. The mRNA and protein expression levels of K-acetyltransferase 2B (KAT2B), general control non-repressed protein 5 (GCN5), histone deacetylase 1 (HDAC1), and histone deacetylase 3 (HDAC3) in the colon were determined using real-time quantitative reverse transcription polymerase chain reaction and immunofluorescence, respectively.</p> Results <p>Compared to the control group, the histopathological score, serum levels of TNF-α, IL-1β, and IL-6, and the mRNA and protein expression of GCN5, HDAC1, and HDAC3 in the colon were significantly increased in the model group (<i>P</i>&lt;0.01 or <i>P</i>&lt;0.05), whereas the protein expression of H3K27ac and the mRNA and protein expression of KAT2B were significantly decreased in the model group (<i>P</i>&lt;0.01 or <i>P</i>&lt;0.05). In contrast, compared to the model group, the histopathological score, serum levels of TNF-α, IL-1β, and IL-6, and the mRNA and protein expression of GCN5, HDAC1, and HDAC3 in the colon were significantly decreased in the herb-insulated moxibustion group (<i>P</i>&lt;0.01 or <i>P</i>&lt;0.05), whereas the protein expression of H3K27ac and the mRNA and protein expression of KAT2B were significantly increased in the model group (<i>P</i>&lt;0.01 or <i>P</i>&lt;0.05). No significant differences in the aforementioned indicators were observed between the sulfasalazine group and the herb-insulated moxibustion group.</p> Conclusion <p>Moxibustion alleviates colonic inflammation in CD rats, potentially by up-regulating the expression of KAT2B, down-regulating the expression of GCN5, HDAC1, and HDAC3, and consequently increasing the H3K27ac level.</p>

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Study on the regulation effect of moxibustion on colonic H3K27 acetylation in rats with Crohn disease

  • 期刊论文

  • 发表日期：

    2026年1月7日

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  • Qin Qi,
  • Liming Chen,
  • Huangan Wu,
  • Yaying Lin,
  • Guona Li,
  • Handan Zheng,
  • Lu Zhu,
  • Rui Zhong,
  • Yan Huang,
  • Luyi Wu,
  • Zhihui Deng,
  • Zhe Ma

摘要

Objective

To investigate the regulatory effect of moxibustion on rats with Crohn disease (CD) and explore the relationship between this effect and alterations in colonic histone H3 lysine 27 acetylation (H3K27ac).

Methods

Ten out of 36 Sprague-Dawley rats were randomly selected as the control group using a random number table method. The remaining 26 rats received an enema of 2,4,6-trinitrobenzene sulfonic acid to establish a CD model. Successfully modeled rats were then randomly divided into a model group, an herb-insulated moxibustion group, and a sulfasalazine group, with 8 rats in each group. The herb-insulated moxibustion group received herb-insulated moxibustion at Qihai (CV6) and bilateral Tianshu (ST25), and the sulfasalazine group received sulfasalazine via gavage. Histopathological changes in the colonic tissue were assessed using hematoxylin-eosin staining. The serum levels of inflammatory factors [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] were measured using the enzyme-linked immunosorbent assay. The protein expression of colonic H3K27ac was detected using Western blotting and immunohistochemistry. The mRNA and protein expression levels of K-acetyltransferase 2B (KAT2B), general control non-repressed protein 5 (GCN5), histone deacetylase 1 (HDAC1), and histone deacetylase 3 (HDAC3) in the colon were determined using real-time quantitative reverse transcription polymerase chain reaction and immunofluorescence, respectively.

Results

Compared to the control group, the histopathological score, serum levels of TNF-α, IL-1β, and IL-6, and the mRNA and protein expression of GCN5, HDAC1, and HDAC3 in the colon were significantly increased in the model group (P<0.01 or P<0.05), whereas the protein expression of H3K27ac and the mRNA and protein expression of KAT2B were significantly decreased in the model group (P<0.01 or P<0.05). In contrast, compared to the model group, the histopathological score, serum levels of TNF-α, IL-1β, and IL-6, and the mRNA and protein expression of GCN5, HDAC1, and HDAC3 in the colon were significantly decreased in the herb-insulated moxibustion group (P<0.01 or P<0.05), whereas the protein expression of H3K27ac and the mRNA and protein expression of KAT2B were significantly increased in the model group (P<0.01 or P<0.05). No significant differences in the aforementioned indicators were observed between the sulfasalazine group and the herb-insulated moxibustion group.

Conclusion

Moxibustion alleviates colonic inflammation in CD rats, potentially by up-regulating the expression of KAT2B, down-regulating the expression of GCN5, HDAC1, and HDAC3, and consequently increasing the H3K27ac level.