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<p>To address critical challenges of protein template denaturation caused by intense exothermicity and prolonged reaction time when using the traditional protein molecular imprinting technology, a novel imprinting strategy was proposed. This study successfully achieved the rapid and controllable <i>in-situ</i> synthesis of polyacrylamide/calcium alginate (PAM/CaAlg) hydrogel films under near-ambient temperature conditions, employing a silver ions (Ag<sup>+</sup>)-catalyzed ammonium persulfate–sodium bisulfite redox system with acrylamide (AM) as the monomer, N,N′-methylenebisacrylamide (MBA) as the crosslinker, and bovine serum albumin (BSA) as the template. The optimized molecularly imprinted polymer (MIP) films demonstrated substantial enhancement of the BSA adsorption capacity following the removal of templates, reaching a maximum equilibrium adsorption capacity (<i>Q</i><sub>e</sub>) of 50.4 mg·g<sup>−1</sup> while maintaining a stable imprinting efficiency (IE) of 2.7. Competitive adsorption experiments verified the exceptional selectivity of MIP films towards the BSA recognition. Additionally, the incorporation of Ag<sup>+</sup> ions endowed both MIP and non-imprinted polymer (NIP) films with remarkable antibacterial properties. This work establishes a straightforward and effective methodology for developing advanced protein-imprinted hydrogels that simultaneously exhibit high adsorption capacity, superior selectivity, and significant antibacterial activity.</p>

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Silver ion-catalysis synthesized protein imprinted polyacrylamide/calcium alginate hydrogel film with high adsorption, recognition, and antibacterial properties

  • 期刊论文

  • 发表日期：

    2025年12月2日

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  • Yixin Li,
  • Yuansheng Lin,
  • Zhihui Han,
  • Minglin Wang,
  • Xianmei Ma,
  • Congcong Liu,
  • Zhilong Guo,
  • Kongyin Zhao,
  • Bohong Kan

摘要

To address critical challenges of protein template denaturation caused by intense exothermicity and prolonged reaction time when using the traditional protein molecular imprinting technology, a novel imprinting strategy was proposed. This study successfully achieved the rapid and controllable in-situ synthesis of polyacrylamide/calcium alginate (PAM/CaAlg) hydrogel films under near-ambient temperature conditions, employing a silver ions (Ag+)-catalyzed ammonium persulfate–sodium bisulfite redox system with acrylamide (AM) as the monomer, N,N′-methylenebisacrylamide (MBA) as the crosslinker, and bovine serum albumin (BSA) as the template. The optimized molecularly imprinted polymer (MIP) films demonstrated substantial enhancement of the BSA adsorption capacity following the removal of templates, reaching a maximum equilibrium adsorption capacity (Qe) of 50.4 mg·g−1 while maintaining a stable imprinting efficiency (IE) of 2.7. Competitive adsorption experiments verified the exceptional selectivity of MIP films towards the BSA recognition. Additionally, the incorporation of Ag+ ions endowed both MIP and non-imprinted polymer (NIP) films with remarkable antibacterial properties. This work establishes a straightforward and effective methodology for developing advanced protein-imprinted hydrogels that simultaneously exhibit high adsorption capacity, superior selectivity, and significant antibacterial activity.