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<p>A novel benzothiazole–naphthalene conjugated probe <b>BMHMA</b> was rationally synthesized and characterized for selective Al<sup>3+</sup> detection. In DMF/H<sub>2</sub>O (1:1, v/v), BMHMA exhibited an approximately 190-fold fluorescence enhancement with a distinct naked-eye color change from dark blue to bright blue upon Al<sup>3+</sup> addition. A 1:1 binding stoichiometry was verified by Job’s plot, offering a low LOD of 11.8&#xa0;nM. Notably, <b>BMHMA</b> enabled reliable Al<sup>3+</sup> determination in real water samples, portable paper-based assays, and cellular imaging. This work provides a high-performance visualizable probe with promising practical potential for environmental monitoring and biological Al<sup>3+</sup> analysis.</p>

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A significant fluorescence turn-on and UV-ratiometric detection of Al3+ and its application in real water samples and live-cell imaging

  • 期刊论文

  • 发表日期：

    2026年5月11日

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  • Guimei Huang,
  • Guiying Hu,
  • Yanfei Qin,
  • Zhiqian Liang,
  • Zhiyong Xing,
  • Yuting Ban,
  • Chaoli Liao,
  • Chuanbin Fan

摘要

A novel benzothiazole–naphthalene conjugated probe BMHMA was rationally synthesized and characterized for selective Al3+ detection. In DMF/H2O (1:1, v/v), BMHMA exhibited an approximately 190-fold fluorescence enhancement with a distinct naked-eye color change from dark blue to bright blue upon Al3+ addition. A 1:1 binding stoichiometry was verified by Job’s plot, offering a low LOD of 11.8 nM. Notably, BMHMA enabled reliable Al3+ determination in real water samples, portable paper-based assays, and cellular imaging. This work provides a high-performance visualizable probe with promising practical potential for environmental monitoring and biological Al3+ analysis.