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<p>A deficiency of L-aspartic acid (L-asp) can cause some problems, including depression, fatigue, low mood, and injury to the nervous system. L-asp improves the parameters of seminal fluid and is effective in the treatment of male infertility. Despite the known significance of L-asp in health, existing detection methods are often complex and costly, limiting their practical applications. Previous relevant studies have not adequately addressed L-asp detection, highlighting a gap in accessible and effective biosensors. This work focused on detecting L-asp using sensitive, cheap, and straightforwardly developed surface-enhanced Raman scattering (SERS) sensors. After the fabrication of AgNPs by a chemical reduction method and characterization of them, a linear range of 10<sup>−1</sup> to 10<sup>−9</sup> M of L-asp was analyzed using Raman spectroscopy, and the density functional theory (DFT) method was employed to confirm the outcomes. The limit of detection (LOD) of the developed substrates for identifying L-asp was 10<sup>− 9</sup> M. Moreover, enhancement factors (EFs) were calculated to be 1.410 × 10<sup>8</sup> and 2.224 × 10<sup>8</sup> using experimental results and finite-difference time-domain (FDTD) simulation, respectively. These straightforward, inexpensive, and thoroughly developed substrates can serve as sensitive biosensors for various applications.</p>

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Detection of trace L-aspartic acid using simple and cost-effective biosensors: experimental study along with density functional theory (DFT) and finite difference time domain (FDTD) numerical investigations

  • 期刊论文

  • 发表日期：

    2025年12月3日

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  • Hossein Sahbafar,
  • Atefe Mohsennezhad,
  • Leila Zeinalizad,
  • Hadis Jamshidvand,
  • Saeideh Mehmandoust,
  • Vahid Eskandari

摘要

A deficiency of L-aspartic acid (L-asp) can cause some problems, including depression, fatigue, low mood, and injury to the nervous system. L-asp improves the parameters of seminal fluid and is effective in the treatment of male infertility. Despite the known significance of L-asp in health, existing detection methods are often complex and costly, limiting their practical applications. Previous relevant studies have not adequately addressed L-asp detection, highlighting a gap in accessible and effective biosensors. This work focused on detecting L-asp using sensitive, cheap, and straightforwardly developed surface-enhanced Raman scattering (SERS) sensors. After the fabrication of AgNPs by a chemical reduction method and characterization of them, a linear range of 10−1 to 10−9 M of L-asp was analyzed using Raman spectroscopy, and the density functional theory (DFT) method was employed to confirm the outcomes. The limit of detection (LOD) of the developed substrates for identifying L-asp was 10− 9 M. Moreover, enhancement factors (EFs) were calculated to be 1.410 × 108 and 2.224 × 108 using experimental results and finite-difference time-domain (FDTD) simulation, respectively. These straightforward, inexpensive, and thoroughly developed substrates can serve as sensitive biosensors for various applications.