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<p>Nanozymes have been valuable tools in colorimetric analysis due to their ease of production, long-term stability, and adaptable enzymatic activity, enabling them to induce color changes in substrates. This study presents a straightforward enzyme-based colorimetric sensor for morphine (<b>MOR</b>) detection. The sensor utilized the laccase-like activity of a self-synthesized Cu, Bi-phosphotungstic acid (<b>Cu, Bi-PTs</b>) nanozyme, which was loaded with phosphotungstic acid, copper, and bismuth through a one-pot microwave method. This nanozyme was capable of catalyzing the coupling reaction of 2,4-dichlorophenol (<b>2,4-DP</b>) and 4-aminoantipyrine (<b>4-AP</b>), leading to the conversion of colorless phenolic compounds into red-colored products. The method demonstrated a detection limit of 1.88&#xa0;μg/mL for <b>MOR</b> and a linear dynamic range from 3.333 to 400&#xa0;μg/mL. The calibration curve, given by the equation y = 0.0015x + 0.4081, exhibited excellent linearity (R<sup>2</sup> = 0.9914), confirming the method's reliability for quantitative analysis. The developed colorimetric method was successfully applied to real samples, showing relative standard deviations (RSD) between 1.4 and 4.3% for <b>MOR</b> detection. These results demonstrated that the method was sensitive, cost-effective, and rapid, making it suitable for the efficient determination of <b>MOR</b>.</p>

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Excellent laccase mimic activity of bimetallic Cu, Bi-PTs nanozyme sensor and its applications in quantification of morphine

  • 期刊论文

  • 发表日期：

    2025年11月24日

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  • Jinwen Zhao,
  • Le Wang,
  • Xiaolan Liu,
  • Yanqin Zhu,
  • Qinhong Yin

摘要

Nanozymes have been valuable tools in colorimetric analysis due to their ease of production, long-term stability, and adaptable enzymatic activity, enabling them to induce color changes in substrates. This study presents a straightforward enzyme-based colorimetric sensor for morphine (MOR) detection. The sensor utilized the laccase-like activity of a self-synthesized Cu, Bi-phosphotungstic acid (Cu, Bi-PTs) nanozyme, which was loaded with phosphotungstic acid, copper, and bismuth through a one-pot microwave method. This nanozyme was capable of catalyzing the coupling reaction of 2,4-dichlorophenol (2,4-DP) and 4-aminoantipyrine (4-AP), leading to the conversion of colorless phenolic compounds into red-colored products. The method demonstrated a detection limit of 1.88 μg/mL for MOR and a linear dynamic range from 3.333 to 400 μg/mL. The calibration curve, given by the equation y = 0.0015x + 0.4081, exhibited excellent linearity (R2 = 0.9914), confirming the method's reliability for quantitative analysis. The developed colorimetric method was successfully applied to real samples, showing relative standard deviations (RSD) between 1.4 and 4.3% for MOR detection. These results demonstrated that the method was sensitive, cost-effective, and rapid, making it suitable for the efficient determination of MOR.