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<p>This work was focused on developing a stable, selective, and sensitive electrochemical aptasensor based on 3,4,9,10-perylene tetracarboxylic acid-functionalized chemically converted graphene (PTCA/CCG)-modified electrode for monitoring neuron-specific enolase (NSE), lung-specific biomarkers. The electrochemical aptasensor relied on the purified NSE aptamer immobilized on PTCA/CCG-modified electrode. The PTCA/CCG promoted both the immobilization of the aptamer and the electrochemical signal. The aptasensor exhibited a low limit of detection (0.008&#xa0;ng/mL), a broad linear range (10<sup>–3</sup> to 10<sup>3</sup>&#xa0;ng/mL), remarkable specificity, excellent repeatability and long-term stability, maintaining initial resistance at 96.57% after 30&#xa0;days. Results exhibited recoveries ranging from 98.00% to 99.00% and relative standard deviation less than 4.08%, demonstrating the acceptable precision and reliability of the fabricated aptasensor and confirming the aptasensor’s performance and efficiency in complex biological fluids and clinical applications.</p>

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Fabrication of aptasensor for monitoring cancer biomarkers: detection of lung-specific biomarkers using chemically converted graphene

  • 期刊论文

  • 发表日期：

    2025年9月29日

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  • Xiuxiu Wang,
  • Jiqun Geng,
  • Hui Jiang,
  • Dadeng Gao,
  • Jun Che,
  • Minwei Gu,
  • Mingjin Xu

摘要

This work was focused on developing a stable, selective, and sensitive electrochemical aptasensor based on 3,4,9,10-perylene tetracarboxylic acid-functionalized chemically converted graphene (PTCA/CCG)-modified electrode for monitoring neuron-specific enolase (NSE), lung-specific biomarkers. The electrochemical aptasensor relied on the purified NSE aptamer immobilized on PTCA/CCG-modified electrode. The PTCA/CCG promoted both the immobilization of the aptamer and the electrochemical signal. The aptasensor exhibited a low limit of detection (0.008 ng/mL), a broad linear range (10–3 to 103 ng/mL), remarkable specificity, excellent repeatability and long-term stability, maintaining initial resistance at 96.57% after 30 days. Results exhibited recoveries ranging from 98.00% to 99.00% and relative standard deviation less than 4.08%, demonstrating the acceptable precision and reliability of the fabricated aptasensor and confirming the aptasensor’s performance and efficiency in complex biological fluids and clinical applications.