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<p>In this study, a reliable fluorescence nanoprobe utilizing bovine serum albumin-modified silver nanocrystals (BSA-modified Ag NCs) was developed for the sensitive quantification of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) in the milk samples. The successful synthesis of BSA-modified Ag NCs was examined through transmission electron microscopy, scanning electron microscopy, fluorescence spectroscopy, energy-dispersive spectrometry, dynamic light scattering, X-ray powder diffraction, and attenuated total reflectance–Fourier transforms infrared. The probe exhibited a significant decrease in fluorescence intensity when exposed to H<sub>2</sub>O<sub>2</sub>, indicating an oxidative interaction between BSA-modified Ag NCs and H<sub>2</sub>O<sub>2</sub>. The proposed nanoprobe displayed a linear response toward H<sub>2</sub>O<sub>2</sub> in the concentration range of 1.5–294&#xa0;nmol/L, with a detection limit of 0.3&#xa0;nmol/L.&#xa0;The intraday precision and inter-day precision which are percentages of relative standard deviation for five repetitive measurements of H<sub>2</sub>O<sub>2</sub> at a concentration of 59&#xa0;nmol/L, respectively, on the same day and different days were 3.4% and 6.8%. The validated method was successfully used for the determination of H<sub>2</sub>O<sub>2</sub> in six milk samples obtained from the local market. This method exhibited high sensitivity and specificity, offering a sensing platform that is simple, convenient, and quick to implement.</p>

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A feasible fluorescent probe based on modified silver nanocrystals for quantification of hydrogen peroxide in milk samples

  • 期刊论文

  • 发表日期：

    2025年9月3日

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  • Kosar Shirazi,
  • Zahra Karimzadeh,
  • Elaheh Rahimpour,
  • Abolghasem Jouyban

摘要

In this study, a reliable fluorescence nanoprobe utilizing bovine serum albumin-modified silver nanocrystals (BSA-modified Ag NCs) was developed for the sensitive quantification of hydrogen peroxide (H2O2) in the milk samples. The successful synthesis of BSA-modified Ag NCs was examined through transmission electron microscopy, scanning electron microscopy, fluorescence spectroscopy, energy-dispersive spectrometry, dynamic light scattering, X-ray powder diffraction, and attenuated total reflectance–Fourier transforms infrared. The probe exhibited a significant decrease in fluorescence intensity when exposed to H2O2, indicating an oxidative interaction between BSA-modified Ag NCs and H2O2. The proposed nanoprobe displayed a linear response toward H2O2 in the concentration range of 1.5–294 nmol/L, with a detection limit of 0.3 nmol/L. The intraday precision and inter-day precision which are percentages of relative standard deviation for five repetitive measurements of H2O2 at a concentration of 59 nmol/L, respectively, on the same day and different days were 3.4% and 6.8%. The validated method was successfully used for the determination of H2O2 in six milk samples obtained from the local market. This method exhibited high sensitivity and specificity, offering a sensing platform that is simple, convenient, and quick to implement.